PMID: 6990552Jan 1, 1980Paper

Chemical carcinogen in vitro testing: a method for sizing cell nuclei in the nuclear enlargement assay

Toxicology
R A FinchH B Bosmann

Abstract

The observation that cells often respond to carcinogens by nuclear enlargement has suggested that this property might be useful to develop a short-term screening test for such compounds. Previous methods for detecting nuclear size increases have used an image analyzer system to detect nuclear changes in individual cells. This paper details a more rapid method for obtaining nuclei by use of a stromalyzing procedure following by analysis of nuclear volumes, using a Coulter Counter Channelyzer. This new and simplified nuclear sizing method should facilitate the use of the assay as a possible carcinogenesis screen by permitting rapid and efficient testing of large numbers of compounds.

References


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Citations

Jan 1, 1991·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A J FowlieP Grasso
Oct 1, 1994·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C WestmorelandP Grasso
Jan 9, 2013·Journal of Agricultural and Food Chemistry·David J Smith, Robin C Anderson
Oct 15, 1982·Biochemical and Biophysical Research Communications·G A Clawson, E A Smuckler
Oct 14, 2016·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·Kenji TakeshitaToshinari Maeda

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