Chemical characterization of Pleurotus eryngii polysaccharide and its tumor-inhibitory effects against human hepatoblastoma HepG-2 cells

Carbohydrate Polymers
Daoyuan RenXingbin Yang

Abstract

This study was designed to investigate the chemical characterization and antitumor effects of Pleurotus eryngii polysaccharides (PEP). The crude PEP was fractionated into two fractions, namely PEP-1 and PEP-2. HPLC analysis showed that PEP-1 and PEP-2 were heteropolysaccharides mainly composed of glucose with the average molecular weights of 2.54×10(4)Da (PEP-1) and 4.63×10(5)Da (PEP-2), respectively. High molecular mass PEP-2 was shown to exhibit stronger growth inhibition against human hepatoblastoma HepG-2 cells in comparison with PEP-1. Flow cytometric analysis showed that PEP-2 exerted a stimulatory effect on apoptosis of HepG-2 cells, and induced the cell-cycle arrest at the S-phase, with the observation of intracellular ROS production. These findings suggest that the polysaccharides, especially PEP-2, are very important nutritional ingredients responsible for the anticancer health benefits of P. eryngii.

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Citations

Jul 9, 2016·International Journal of Biological Macromolecules·Sun LipingZhuang Yongliang
Nov 5, 2016·International Journal of Biological Macromolecules·Li ChenShujun Wang
Jun 5, 2018·Food & Function·Chaima BouzgarrouIsabel C F R Ferreira
Aug 21, 2019·International Journal of Molecular Sciences·Adriana FontesAnabela Marisa Azul
Jan 13, 2021·International Journal of Biological Macromolecules·Chen ZhangQihang Yang
Jun 3, 2021·Journal of Fungi·Allen Grace NiegoKevin D Hyde
May 18, 2021·Journal of Food Biochemistry·Aparajita SharmaAstha Tripathi
Sep 16, 2019·Carbohydrate Polymers·Ruberney S OliveiraElaine R Carbonero
Apr 17, 2018·Journal of Agricultural and Food Chemistry·Li Qiao LiuMing Yong Xie
Aug 20, 2021·International Journal of Biological Macromolecules·Hui XuWenjian Yang

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