PMID: 702646Oct 1, 1978Paper

Chemical cross-linking of proteins of Semliki Forest virus: virus particles and plasma membranes from BHK-21 cells treated with colchicine or dibucaine

Journal of Virology
C D Richardson, D E Vance

Abstract

Chemical cross-linking of the proteins of Semliki Forest virus has been performed in virus particles and in baby hamster kidney-21 (BHK-21) cells infected with Semliki Forest virus. Most of the studies were done with the reversible cross-linkers dimethyl 3,3'-thiobis(propionimidate) and dithiobis(succinimidyl propionate). The identity of the cross-linked species was determined by two-dimensional electrophoresis. The results with virus particles showed extensive cross-linking of the nucleocapsid proteins and the formation of dimers of the two large envelope glycoproteins (E1 and E2). Similar patterns for the cross-linked virus proteins were observed in plasma membranes isolated from BHK-21 cells infected with Semliki Forest virus. No cross-linking of the third envelope glycoprotein (E3) was observed. Also, there was no evidence for significant cross-linking between host and virus proteins. The addition of colchicine, a drug that disrupts microtubules, to infected BHK-21 cells had no effect on the cross-linking of virus proteins in the plasma membrane. In contrast, dibucaine, a local anesthetic, greatly inhibited the formation of envelope dimers (E1-E2) in plasma membranes, but not in virus particles. The implication of these res...Continue Reading

References

Jul 1, 1975·Journal of Virology·C H von Bonsdorff, S C Harrison
Jan 1, 1977·Annual Review of Biochemistry·K Peters, F M Richards
Nov 15, 1973·Journal of Molecular Biology·U K Laemmli, M Favre
Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·H Garoff, K Simons
Jan 1, 1972·The Journal of General Virology·S I Kennedy, D C Burke

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Citations

Jan 1, 1979·CRC Critical Reviews in Biochemistry·E J PatzerE J Dubovi
Jan 1, 1989·Archives of Virology·N J DimmockA R Guest
Sep 4, 2014·Microbiology and Molecular Biology Reviews : MMBR·Ping JiangEckard Wimmer

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