Chemical cross-linking of the substance P (NK-1) receptor to the alpha subunits of the G proteins Gq and G11

Biochemistry
S G MacdonaldN D Boyd

Abstract

We have previously shown that the high-affinity binding of substance P (SP) to its receptor is dependent on an interaction with a PTX-insensitive G protein. This G protein couples SP receptor activation to stimulation of its effector, phospholipase C. In this study, we combined photoaffinity labeling, chemical cross-linking techniques, and immunological characterization using sequence-specific antibody probes to identify G proteins that couple to the SP receptor. First we covalently labeled the SP receptor present on rat submaxillary gland membranes with a radioiodinated photoreactive derivative of SP, p-benzoyl-L-phenylalanine(8)-substance P (125I-[Bpa8]SP). Photoincorporation of this SP derivative was susceptible to guanine nucleotide inhibition, indicating that the receptor was coupled to its G protein during labeling. We then used a chemical cross-linking agent to covalently link the photoaffinity labeled SP receptor and its associated G protein. Cross-linking generated a 96 kDa product, formation of which was prevented by the addition of a guanine nucleotide, but not an adenine nucleotide, following photolabeling, but prior to cross-linking. Furthermore, the 96 kDa cross-linked complex was absent in membranes which had bee...Continue Reading

Citations

Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·M InoueH Ueda
Oct 28, 2009·The Journal of General Physiology·John F HeneghanAnn R Rittenhouse
Mar 11, 1998·Journal of Receptor and Signal Transduction Research·K NishimuraM M Kwatra
Jun 18, 1998·British Journal of Pharmacology·T V LewisJ P Chin-Dusting
Sep 27, 2003·Journal of Neurochemistry·F LallemendB Malgrange
Nov 25, 2005·Autonomic Neuroscience : Basic & Clinical·Hikma Boutaghou-CheridMarie-Odile Christen
Oct 1, 2015·BioMed Research International·Susana Garcia-Recio, Pedro Gascón
Mar 7, 2002·Experimental Eye Research·Limin QianAustin K Mircheff
Mar 25, 2015·Cell Reports·Shanna L BowmanManojkumar A Puthenveedu
Apr 3, 2019·International Journal of Molecular Sciences·Chu-Ting ChangChih-Cheng Chen
May 16, 2012·Proceedings of the National Academy of Sciences of the United States of America·John E LinleyNikita Gamper
Sep 11, 2014·Pflügers Archiv : European journal of physiology·Yamhilette LiconUlises Meza
Mar 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael T BorchersJames J Lee
Nov 9, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·B L FiebichK Lieb
Jun 5, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Selena Wei Shan SioMadhav Bhatia
Aug 12, 1999·European Journal of Pharmacology·A Saria

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.