Chemical cross-linking of xenopericardial biomeshes: A bottom-up study of structural and functional correlations

Xenotransplantation
Ekaterina A GrebenikPeter S Timashev

Abstract

Decellularized bovine pericardium (DBP)-based biomeshes are the gold standard in reconstructive surgery. In order to prolong their stability after the transplantation, various chemical cross-linking strategies are employed. However, structural and functional properties of the biomeshes differ in dependence on the cross-linker used. Here, we performed a bottom-up study of structural and functional alterations of DBP-based biomeshes following cross-linking with hexamethylene diisocyanate (HMDC), ethylene glycol diglycidyl ether (EGDE), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and genipin. The in vitro cytotoxicity tests supported their clinical applicability. Their structural differences (eg roughness, fibre thickness, pore morphology) were evaluated using the two-photon confocal laser scanning, atomic force, scanning electron and polarized light microscopies. HMDC and EDC samples appeared to be the roughest. Complex mechanical trials indicated the tendency to reduced Young's Modulus and mechanical anisotropy values of DBP upon cross-linking. The lowest mechanical anisotropy was found in EDC and genipin sample groups. In vitro collagenase susceptibility was the highest for EDC samples and the lowest for EGDE samples. T...Continue Reading

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Citations

Feb 23, 2020·Biotechnology Journal·Ekaterina A GrebenikPeter S Timashev
Mar 7, 2020·Frontiers in Bioengineering and Biotechnology·Vadim ElaginPeter Timashev
Sep 2, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Elvira R GafarovaPeter S Timashev
Apr 7, 2020·Journal of Biomaterials Science. Polymer Edition·Ilyas InciHuseyin Avci
Aug 9, 2019·Xenotransplantation·Nicole Forneris, Christopher Burlak
Mar 2, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Ali ShiraniAli Samadikuchaksaraei
Aug 15, 2021·Journal of Biomedical Materials Research. Part a·Irina Yu ZhuravlevaNatalia R Nichay

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