Chemical cytometry: fluorescence-based single-cell analysis

Annual Review of Analytical Chemistry
Daniella CohenNorman J Dovichi

Abstract

Cytometry deals with the analysis of the composition of single cells. Flow and image cytometry employ antibody-based stains to characterize a handful of components in single cells. Chemical cytometry, in contrast, employs a suite of powerful analytical tools to characterize a large number of components. Tools have been developed to characterize nucleic acids, proteins, and metabolites in single cells. Whereas nucleic acid analysis employs powerful polymerase chain reaction-based amplification techniques, protein and metabolite analysis tends to employ capillary electrophoresis separation and ultrasensitive laser-induced fluorescence detection. It is now possible to detect yoctomole amounts of many analytes in single cells.

References

Jul 1, 1976·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R Rüchel
Oct 6, 1989·Science·R T KennedyJ W Jorgenson
Oct 1, 1966·Analytical Biochemistry·H HydénB McEwen
Nov 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·J J Marchalonis, G J Nossal
Dec 31, 1965·Science·G T Matioli, H B Niewisch
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·J GaoG M Whitesides
Dec 13, 1996·Journal of Chromatography. B, Biomedical Applications·S J Lillard, E S Yeung
Apr 15, 1997·Analytical Chemistry·G J OpiteckR J Anderegg
Feb 10, 1998·Electrophoresis·N J Dovichi
Aug 13, 1998·Journal of Neuroscience Methods·V ParpuraP G Haydon
Oct 9, 1998·Journal of Chromatography. a·A M Ho, E S Yeung
Aug 3, 1999·Nature Biotechnology·C L LeeN L Allbritton
Sep 16, 1999·Journal of Chromatography. a·D P RichardsN J Dovichi
Feb 5, 2000·Nature Biotechnology·S S RubakhinJ V Sweedler
Feb 5, 2000·Analytical Chemistry·Z ZhangN J Dovichi
Mar 4, 2000·Nature Biotechnology·G D MeredithN L Allbritton
Mar 4, 2000·Analytical Chemistry·S N KrylovN J Dovichi
Mar 31, 2000·Trends in Biotechnology·L LiJ V Sweedler
Jun 6, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·S N KrylovN J Dovichi
Sep 15, 2000·Journal of Immunological Methods·N Baumgarth, M Roederer
Mar 29, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·S HuN J Dovichi
Sep 27, 2001·Journal of Chromatography. a·H LiuS A Cohen
Mar 30, 2002·Nature Biotechnology·Christoph A KleinGert Riethmüller
Jul 4, 2002·Molecular & Cellular Proteomics : MCP·David A MichelsNorman J Dovichi

❮ Previous
Next ❯

Citations

Apr 13, 2010·Analytical and Bioanalytical Chemistry·Daniel T Chiu
Jun 4, 2010·Analytical and Bioanalytical Chemistry·Shencheng GeChristy L Haynes
Jun 15, 2010·Analytical and Bioanalytical Chemistry·Andrea AmantonicoRenato Zenobi
May 8, 2010·Analytical Chemistry·Xin XuEdgar A Arriaga
Apr 20, 2011·Analytical Chemistry·Yuqing LinAndrew G Ewing
Apr 6, 2011·Nature Methods·Stanislav S RubakhinJonathan V Sweedler
Feb 18, 2011·Lab on a Chip·K Scott PhillipsNancy L Allbritton
May 4, 2010·Annual Review of Biomedical Engineering·Richard N Zare, Samuel Kim
Jul 19, 2008·Annual Review of Analytical Chemistry·Laura M BorlandNancy L Allbritton
Mar 28, 2012·The Plant Journal : for Cell and Molecular Biology·Akira Oikawa, Kazuki Saito
Feb 4, 2010·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Karel Klepárník, Petr Boček
Feb 20, 2016·Expert Review of Molecular Diagnostics·Bjørn T GjertsenJörn Skavland
Mar 2, 2016·Annual Review of Biomedical Engineering·Tania KonryNoa Cohen
Nov 1, 2013·Angewandte Chemie·Liangliang SunNorman J Dovichi
Oct 15, 2009·Chemical Communications : Chem Comm·X Z NiuA J deMello
Mar 12, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Xiaowen OuBi-Feng Liu
Dec 22, 2009·Lab on a Chip·Clarissa LuiCarl Batt
Oct 21, 2011·Electrophoresis·Michelle L KovarikNancy L Allbritton
Jun 14, 2012·Lab on a Chip·Kjeld G H JanssenThomas Hankemeier
Jun 14, 2020·Nature Communications·Sahngki HongRenkun Chen
Jan 13, 2011·Angewandte Chemie·Edward S Yeung
Sep 28, 2016·Proceedings of the National Academy of Sciences of the United States of America·Rongrong PanHong-Yuan Chen
Jun 29, 2012·Chembiochem : a European Journal of Chemical Biology·Dietlind AdlercreutzMonica M Palcic
Apr 30, 2021·Biomedical Materials·Shanni HongYi Lu
Jul 14, 2021·Journal of Biomedical Research·Gradimir Misevic
Jul 24, 2021·Analytical Chemistry·Zhichang YangLiangliang Sun

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
conventional cytometry
conventional
chemical cytometry
electrophoresis
electrophoretic profiles
biopsies
fluorescence microscopy
PCR
nucleic acid amplification

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.