PMID: 7544908Sep 1, 1995Paper

Chemical denaturation of ovalbumin abrogates the induction of oral tolerance of specific IgG antibody and DTH responses in mice

Scandinavian Journal of Immunology
H J PengB T Huang

Abstract

We have examined the effects of ingestion of chemically denatured ovalbumin (OVA) in mice. Both 8 M urea-denatured OVA (UD-OVA) and carboxymethylated UD-OVA (CM-OVA) were purified by gel filtration. Specific IgG antibody and systemic delayed-type hypersensitivity (DTH) responses to OVA were not suppressed by CM-OVA fed prior to or after immunization with OVA in complete Freund's adjuvant (CFA). When CM-OVA was used instead of OVA, for immunization, serum IgG and DTH responses to CM-OVA were orally tolerized by OVA, but not by UD-OVA or CM-OVA. Studies of antigen uptake in mice using sandwich ELISA tests showed that OVA, but not CM-OVA, was absorbed after antigen ingestion. In vitro studies further demonstrated that CM-OVA was digested much more rapidly than OVA. Moreover, studies using bovine serum albumin (BSA) demonstrated that both IgG and DTH responses to BSA were orally tolerant to BSA, but not to denatured BSA. Finally, studies using human gamma-globulin (HGG), a well-known tolerogen, also found that the IgG antibody response to HGG was not orally tolerized by denatured HGG. These results suggest that complete denaturation of globular proteins may affect their processing and absorption in the gut and thus abrogates oral t...Continue Reading

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Citations

Mar 3, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S TropY Ilan
Mar 17, 2004·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·A O KamphorstR D Sinisterra
Dec 7, 2007·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Ching-Feng HuangHo-Jen Peng
Jun 19, 2002·The Proceedings of the Nutrition Society·S Strobel
Jun 4, 2011·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Ching-Feng HuangHo-Jen Peng
Jul 10, 2013·Journal of Animal Physiology and Animal Nutrition·U K ShandilyaV K Kansal
Nov 27, 2004·Mechanisms of Ageing and Development·Kohtaro Fujihashi, Jerry R McGhee
Dec 23, 1999·Clinical Immunology : the Official Journal of the Clinical Immunology Society·L Mayer
Aug 12, 1998·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·B StranskyN M Vaz
May 22, 2002·Annals of the New York Academy of Sciences·Stephan Strobel
Feb 13, 1996·Annals of the New York Academy of Sciences·P Brandtzaeg

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