Chemical Genetics Reveals Environment-Specific Roles for Quorum Sensing Circuits in Pseudomonas aeruginosa

Cell Chemical Biology
Michael A Welsh, Helen E Blackwell

Abstract

Nutritional cues differentially influence the activities of the three quorum sensing (QS) circuits-Las, Rhl, and Pqs-in the pathogen Pseudomonas aeruginosa. A full understanding of how these systems work together to tune virulence factor production to the environment is lacking. Here, we used chemical probes to evaluate the contribution of each QS circuit to virulence in wild-type P. aeruginosa under defined environmental conditions. Our results indicate that Rhl and Pqs drive virulence factor production in phosphate- and iron-limiting environments, while Las has a minor influence. Consequently, simultaneous inhibition of Rhl and Pqs can attenuate virulence in environments where Las inhibition fails. The activity trends generated in this study can be extrapolated to predict QS inhibitor activity in infection-relevant environments, such as cystic fibrosis sputum. These results indicate that environmental signals can drastically alter the efficacy of small-molecule QS inhibitors in P. aeruginosa and possibly other pathogens.

References

Nov 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·H CaoL G Rahme
Sep 5, 2002·Nature Reviews. Molecular Cell Biology·Clay Fuqua, E Peter Greenberg
Mar 20, 2003·Journal of Bacteriology·Victoria E WagnerBarbara H Iglewski
Feb 14, 2006·FEMS Microbiology Reviews·Vittorio Venturi
Feb 25, 2006·Science·Andrew Camilli, Bonnie L Bassler
Sep 13, 2006·Antimicrobial Agents and Chemotherapy·Ute MühE Peter Greenberg
Mar 16, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Catharine E White, Stephen C Winans
Apr 24, 2007·Journal of Bacteriology·Kangmin Duan, Michael G Surette
Dec 15, 2007·Nature Reviews. Microbiology·Lynette CegelskiScott J Hultgren
Apr 22, 2008·The Journal of Biological Chemistry·Amanda G OglesbyMichael L Vasil
Aug 6, 2008·Nature Reviews. Microbiology·Stacie A BrownMarvin Whiteley
Sep 2, 2008·Bioorganic & Medicinal Chemistry Letters·Grant D GeskeHelen E Blackwell
Apr 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Alexander ZaborinJohn C Alverdy
Aug 18, 2009·Molecular Microbiology·Kerrigan B GilbertMartin Schuster
Nov 23, 2010·Chemical Reviews·Neri AmaraMichael M Meijler
Apr 19, 2011·Bioresource Technology·Rodrigo S ReisDenise M G Freire
Oct 12, 2011·Proceedings of the National Academy of Sciences of the United States of America·Stefanie LimmerDominique Ferrandon
Sep 6, 2012·Organic & Biomolecular Chemistry·Thanit PraneenararatHelen E Blackwell
Nov 6, 2012·Cold Spring Harbor Perspectives in Medicine·Steven T Rutherford, Bonnie L Bassler
Nov 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·David A RecinosLars E P Dietrich
Nov 13, 2012·Nucleic Acids Research·Deepak BalasubramanianKalai Mathee
Nov 14, 2012·Nature Reviews. Microbiology·Anders FolkessonSøren Molin
Apr 2, 2013·Nature Chemical Biology·Jasmine LeeLian-Hui Zhang
Oct 22, 2013·Proceedings of the National Academy of Sciences of the United States of America·Colleen T O'LoughlinBonnie L Bassler
Jan 17, 2014·Frontiers in Cellular and Infection Microbiology·Panayiota Markou, Yiorgos Apidianakis
Mar 7, 2014·Journal of Medicinal Chemistry·Ewan J MurrayPaul Williams
Mar 15, 2014·Nature Reviews. Microbiology·Richard C AllenSam P Brown
Jul 25, 2014·PLoS Genetics·Keith H TurnerMarvin Whiteley
Sep 25, 2014·Protein & Cell·Jasmine Lee, Lianhui Zhang

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Citations

Mar 19, 2016·Cell Chemical Biology·Michael M Meijler
Jun 15, 2016·Journal of Microbiological Methods·Budoor AlqarniScott A Rice
Aug 12, 2016·Nature Reviews. Microbiology·Kai Papenfort, Bonnie L Bassler
Oct 11, 2016·Chemical Communications : Chem Comm·Dávid SzamosváriThomas Böttcher
Dec 23, 2016·Chembiochem : a European Journal of Chemical Biology·Joseph K VasquezHelen E Blackwell
Aug 30, 2017·Proceedings of the National Academy of Sciences of the United States of America·Brent Cezairliyan, Frederick M Ausubel
Apr 25, 2019·International Journal of Molecular Sciences·Viola Camilla ScoffoneSilvia Buroni
Nov 10, 2018·Beilstein Journal of Organic Chemistry·Matthew J Styles, Helen E Blackwell
Nov 10, 2018·Beilstein Journal of Organic Chemistry·Christian Schütz, Martin Empting
May 28, 2020·Journal of Medical Microbiology·Divakar BadalVarsha Singh
Jan 26, 2017·The Journal of Biological Chemistry·Jon E PaczkowskiBonnie L Bassler
Jun 9, 2016·FEMS Microbiology Reviews·Michael A Welsh, Helen E Blackwell
May 12, 2020·Frontiers in Microbiology·Benjamin RémyÉric Chabrière
Dec 12, 2018·Frontiers in Microbiology·Manuel Alcalde-RicoJosé Luis Martínez
Jun 30, 2016·FEMS Microbiology Reviews·Lisa A HawverWai-Leung Ng
Feb 16, 2021·Frontiers in Cellular and Infection Microbiology·Martha María Juárez-RodríguezIsrael Castillo-Juárez
Feb 13, 2021·Critical Care : the Official Journal of the Critical Care Forum·Yohei MigiyamaTakuro Sakagami
Jul 19, 2018·Progress in Lipid Research·Nishikant WaseConcetta DiRusso
Apr 11, 2021·Applied and Environmental Microbiology·Rhea G AbisadoJosephine R Chandler

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