Chemical hybridizing agent SQ-1-induced male sterility in Triticum aestivum L.: a comparative analysis of the anther proteome

BMC Plant Biology
Hongzhan LiuLili Li

Abstract

Heterosis is widely used to increase the yield of many crops. However, as wheat is a self-pollinating crop, hybrid breeding is not so successful in this organism. Even though male sterility induced by chemical hybridizing agents is an important aspect of crossbreeding, the mechanisms by which these agents induce male sterility in wheat is not well understood. We performed proteomic analyses using the wheat Triticum aestivum L.to identify those proteins involved in physiological male sterility (PHYMS) induced by the chemical hybridizing agent CHA SQ-1. A total of 103 differentially expressed proteins were found by 2D-PAGE and subsequently identified by MALDI-TOF/TOF MS/MS. In general, these proteins had obvious functional tendencies implicated in carbohydrate metabolism, oxidative stress and resistance, protein metabolism, photosynthesis, and cytoskeleton and cell structure. In combination with phenotypic, tissue section, and bioinformatics analyses, the identified differentially expressed proteins revealed a complex network behind the regulation of PHYMS and pollen development. Accordingly, we constructed a protein network of male sterility in wheat, drawing relationships between the 103 differentially expressed proteins and th...Continue Reading

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Citations

Apr 4, 2019·International Journal of Molecular Sciences·Shuping WangGaisheng Zhang

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Datasets Mentioned

BETA
AF069309
AY049040

Methods Mentioned

BETA
protein assay
electrophoresis
PCR
MDA

Software Mentioned

ClueGO
Cytoscape
BiNGO
Multiple Experiment Viewer
PDQuest
Biological Networks Gene Ontology tool ( BiNGO )

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