Chemical ligation methods for the tagging of DNA-encoded chemical libraries

Current Opinion in Chemical Biology
Anthony D KeefeYing Zhang

Abstract

The generation of DNA-encoded chemical libraries requires the unimolecular association of multiple encoding oligonucleotides with encoded chemical entities during combinatorial synthesis processes. This has traditionally been achieved using enzymatic ligation. We discuss a range of chemical ligation methods that provide alternatives to enzymatic ligation. These chemical ligation methods include the generation of modified internucleotide linkages that support polymerase translocation and other modified linkages that while not supporting the translocation of polymerases can also be used to generate individual cDNA molecules containing encoded chemical information specifying individual library members. We also describe which of these approaches have been successfully utilized for the preparation of DNA-encoded chemical libraries and those that were subsequently used for the discovery of inhibitors.

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Citations

Jan 29, 2016·ACS Chemical Biology·Hazem SalamonAndreas Brunschweiger
Feb 26, 2016·Journal of Medicinal Chemistry·Raphael M Franzini, Cassie Randolph
Aug 2, 2016·Drug Discovery Today·Gunther Zimmermann, Dario Neri
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Oct 13, 2020·Chemical Reviews·Patrick R Fitzgerald, Brian M Paegel
Sep 15, 2021·Chembiochem : a European Journal of Chemical Biology·Kazuki YamaokaHiroshi Abe

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