Chemical methods for the simultaneous quantitation of metabolites and proteins from single cells

Journal of the American Chemical Society
Min XueJames R Heath

Abstract

We describe chemical approaches for integrated metabolic and proteomic assays from single cells. Quantitative assays for intracellular metabolites, including glucose uptake and three other species, are designed as surface-competitive binding assays with fluorescence readouts. This enables integration into a microarray format with functional protein immunoassays, all of which are incorporated into the microchambers of a single-cell barcode chip (SCBC). By using the SCBC, we interrogate the response of human-derived glioblastoma cancer cells to epidermal growth factor receptor inhibition. We report, for the first time, on both the intercellular metabolic heterogeneity as well as the baseline and drug-induced changes in the metabolite-phosphoprotein correlation network.

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Citations

Dec 17, 2015·Nature Reviews. Drug Discovery·James R HeathPaul S Mischel
Nov 26, 2015·Proceedings of the National Academy of Sciences of the United States of America·James R Heath
Dec 2, 2015·Analytical Chemistry·Michael G Roper
Nov 17, 2015·Analytical Chemistry·Sanjin HosicAbigail N Koppes
Feb 3, 2016·Technology·Young Shik ShinJames R Heath
Nov 6, 2015·ACS Central Science·James H WadeRyan C Bailey
Jun 9, 2016·Nature Methods·Ahmet F Coskun, Long Cai
Jun 12, 2016·Trends in Biotechnology·Wonmo KangHoracio D Espinosa
Mar 2, 2016·Annual Review of Biomedical Engineering·Tania KonryNoa Cohen
Jul 29, 2016·Nature Protocols·Chi-Chih KangAmy E Herr
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Dec 2, 2017·Chemistry : a European Journal·Manas MondalJia Guo
Mar 17, 2017·Scientific Reports·L KelbauskasDeirdre R Meldrum
Sep 5, 2018·Lab on a Chip·Alexander M XuJames R Heath
Sep 15, 2018·Nanotechnology·Ziyi HePeng Li
Feb 20, 2018·Molecular Cancer·Michaela PoliakováMichaela Medová
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Jul 17, 2021·Journal of the American Chemical Society·Zhili GuoMin Xue
Apr 10, 2018·Analytical Chemistry·Ruihan BaiYao Lu

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