PMID: 8948646Nov 15, 1996Paper

Chemical methods of DNA and RNA fluorescent labeling

Nucleic Acids Research
D Proudnikov, A Mirzabekov

Abstract

Several procedures have been described for fluorescent labeling of DNA and RNA. They are based on the introduction of aldehyde groups by partial depurination of DNA or oxidation of the 3'-terminal ribonucleoside in RNA by sodium periodate. Fluorescent labels with an attached hydrazine group are efficiently coupled with the aldehyde groups and the hydrazone bonds are stabilized by reduction with sodium cyanoborohydride. Alternatively, DNA can be quantitatively split at the depurinated sites with ethylenediamine. The aldimine bond between the aldehyde group in depurinated DNA or oxidized RNA and ethylenediamine is stabilized by reduction with sodium cyanoborohydride and the primary amine group introduced at these sites is used for attachment of isothiocyanate or succinimide derivatives of fluorescent dyes. The fluorescent DNA labeling can be carried out either in solution or on a reverse phase column. These procedures provide simple, inexpensive methods of multiple DNA labeling and of introducing one fluorescent dye molecule per RNA, as well as quantitative DNA fragmentation and incorporation of one label per fragment. These methods of fluorophore attachment were shown to be efficient for use in the hybridization of labeled RNA, ...Continue Reading

References

Feb 1, 1978·Nucleic Acids Research·T R BrokerN Davidson
Jan 1, 1991·DNA Sequence : the Journal of DNA Sequencing and Mapping·K R KhrapkoA D Mirzabekov
Oct 9, 1989·FEBS Letters·K R KhrapkoA D Mirzabekov
Jan 1, 1989·Methods in Enzymology·A D MirzabekovK K Ebralidse
Jan 30, 1987·Biochemical and Biophysical Research Communications·A ReisfeldM Wilchek
Sep 12, 1972·Biochemistry·T Lindahl, B Nyberg
Jan 1, 1980·Methods in Enzymology·A M Maxam, W Gilbert
May 25, 1995·Nucleic Acids Research·P J McHugh, J Knowland
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·A C PeaseS P Fodor
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·G YershovA Mirzabekov
Jun 1, 1960·Proceedings of the National Academy of Sciences of the United States of America·P C ZamecnikJ F Scott

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Citations

Jul 13, 2000·Bioorganic & Medicinal Chemistry Letters·Z WangP F Torrence
Dec 1, 2012·Analytical Chemistry·Vinalia TjongAshutosh Chilkoti
Feb 5, 2013·Journal of the American Chemical Society·Shun NakanoTakashi Morii
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·M F DenissenkoG P Pfeifer
Jun 24, 2011·Journal of Biomolecular Structure & Dynamics·R SerikovM Zenkova
Oct 6, 2000·Journal of Biomolecular Structure & Dynamics·V R ChechetkinA D Mirzabekov
Nov 25, 2000·Nucleosides, Nucleotides & Nucleic Acids·E TrévisiolJ Lhomme
Apr 6, 2006·Oligonucleotides·Tatyana O KabilovaValentin V Vlassov
Jul 29, 2008·Nucleic Acids Research·M van den HoutN H Dekker
Sep 29, 2011·Nucleic Acids Research·John J TurnerSven Klussmann
Apr 23, 2013·Nucleic Acids Research·Weiwei WangYunyu Shi
Oct 31, 2006·Applied and Environmental Microbiology·Wen-Tso LiuJer-Horng Wu
Feb 7, 2001·Applied and Environmental Microbiology·S G BavykinA D Mirzabekov
Mar 27, 2007·Molekuliarnaia biologiia·O A VinogradovaD V Pyshnyĭ
Jul 1, 2010·Molekuliarnaia biologiia·I S KruglovaE L Chernolovskaia
May 21, 2009·Journal of Biomaterials Science. Polymer Edition·Olesya Gusachenko SimonovaMarina A Zenkova
Dec 24, 2005·RNA·Yangming Wang, Scott K Silverman
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·B ChanK Musier-Forsyth
Sep 24, 2013·Organic & Biomolecular Chemistry·Ekaterina A IvanovaMarina A Zenkova
Apr 5, 2002·Human Mutation·Alexander Kolchinsky, Andrei Mirzabekov
Feb 18, 2016·Methods : a Companion to Methods in Enzymology·Fabian Muttach, Andrea Rentmeister
Oct 17, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Patrycja Bielecka, Bernard Juskowiak
Feb 14, 2012·Expert Opinion on Drug Discovery·Wendy A Lea, Anton Simeonov
Dec 6, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mikhail A MaslovMarina A Zenkova
Mar 1, 2011·Methods : a Companion to Methods in Enzymology·Eduardo ParedesSubha R Das
Oct 18, 2014·Journal of the American Chemical Society·Erik D HolmstromDavid J Nesbitt
Sep 30, 2009·Analytica Chimica Acta·Athanasios G Vlessidis, Nicholaos P Evmiridis
Oct 9, 2014·The Journal of Organic Chemistry·Jack L Sloane, Marc M Greenberg
Oct 12, 2013·Angewandte Chemie·Ugo PradèreJonathan Hall

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