PMID: 11330840May 2, 2001Paper

Chemical modification and structural analysis of the progesterone membrane binding protein from porcine liver membranes

Molecular and Cellular Biochemistry
E FalkensteinM Wehling

Abstract

In addition to the classical genomic steroid actions on modulation of transcription and protein synthesis, rapid, nongenomic effects have been described for various steroids. These effects on cellular signaling and function are supposed to be transmitted by membrane binding sites unrelated to the classical intracellular receptors. Recently, a high affinity progesterone membrane binding protein (mPR) has been characterized in porcine liver membranes. In the present study, amino acid residues that are essential for progesterone binding to porcine liver microsomal mPR have been identified by the use of protein modifying reagents. Among all reagents tested, agents with specificity for carboxyl groups, methionine and tryptophan such as N,N'-dicyclohexylcarbodiimide, chloramine T and N-bromosuccinimide induced a reduction in [3H]progesterone binding. To evaluate the presence of essential disulfide bridges, porcine liver microsomes were incubated with the disulfide reducing agent dithiothreitol (DTT) and [3H]progesterone binding was measured. This treatment also resulted in a reduction of binding activity with an IC50 of 20 mM for DTT. Western-blotting analysis in the presence or absence of the reducing agent suggested that mPR--in it...Continue Reading

Citations

Sep 21, 2006·The Journal of Clinical Endocrinology and Metabolism·Lawrence EngmannJohn J Peluso
Sep 3, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Maeilena KampaElias Castanas
Mar 6, 2008·The Journal of Clinical Endocrinology and Metabolism·John J PelusoKathryn Phoenix
Jul 5, 2003·Physiological Reviews·Ralf M LoselMartin Wehling
Nov 23, 2007·Biochemistry·Alisha M ThompsonTheodore R Holman
Jul 27, 2002·Domestic Animal Endocrinology·T A BramleyG Scobie
May 12, 2004·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Michael SchumacherAlejandro F De Nicola
May 27, 2021·Endocrinology·Lindsey S Treviño, Daniel A Gorelick

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