PMID: 2107876Mar 29, 1990Paper

Chemical modification of erythropoietin: an increase in in vitro activity by guanidination

Biochimica Et Biophysica Acta
R SatakeK Asano

Abstract

Human recombinant erythropoietin (rHuEPO) was chemically modified with several group-specific reagents in order to study the role of each kind of amino-acid residue in its biological activity. Guanidination of the amino groups of the lysine residues yielded derivatives that showed higher activities in vitro than native rHuEPO, whereas amidination had no effect on the activity. By contrast, modification of the positive charges of the lysine residues to neutral or negative charges, such as in carbamylation, trinitrophenylation, acetylation or succinylation, caused a significant loss of rHuEPO activity. Chemical modification of other amino-acid residues, such as arginine and tyrosine residues or carboxyl groups, also led to loss of activity.

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Jul 31, 2012·Critical Care : the Official Journal of the Critical Care Forum·Nimesh S A PatelChristoph Thiemermann
Dec 21, 2011·Molecular Medicine·Michael Brines, Anthony Cerami
Dec 12, 2012·Clinical and Experimental Nephrology·Kyubok Jin
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