Chemical modification of metallic implant surfaces with biofunctional proteins (Part 1). Molecular structure and biological activity of a modified NiTi alloy surface

Dental Materials Journal
K Endo

Abstract

Human plasma fibronectin (pFN), an adhesive protein, was covalently immobilized onto an alkylaminosilane derivative of a NiTi substrate with glutaraldehyde through Schiff's base formation. The surface at different stages of the modification was characterized by X-ray photoelectron spectroscopy (XPS), and the amount of immobilized pFN was determined by a fluorometric method. The spreading behavior of human gingival fibroblasts was examined on the modified surface. The XPS spectra suggested that gamma-aminopropyltriethoxysilane (gamma-APS) was bonded to the surface through metallosiloxane bonds (Ti-O-Si) formed via a condensation reaction between the silanol end of gamma-APS and the surface hydroxyl group, with a highly cross-linked siloxane network formed after heat treatment of the silanized surface at 100 degrees C. The pFN was immobilized at the surface density of 1.1 micrograms.cm-2, and significantly promoted fibroblast spreading, suggesting that this chemical modification offers an effective means of controlling metal/cell interactions. These results may contribute to the development of bioactive metallic implants.

Citations

Jan 1, 2012·Journal of Functional Biomaterials·Maria G RaucciLuigi Ambrosio
Jul 27, 2017·Journal of Materials Science. Materials in Medicine·Deanna IngrassiaGiuseppe Maria de Peppo
Oct 20, 2006·Journal of Biomedical Materials Research. Part a·C Krishna PrasadLissy K Krishnan
Mar 4, 2014·Dental Materials Journal·Takuma SuzukiKazuhiro Gomi
Apr 22, 2015·The Open Biomedical Engineering Journal·X LinY Liu
Sep 11, 2020·Dental Materials Journal·Yuki IchiokaKazuhiko Endo
Oct 21, 2003·Journal of Biomedical Materials Research. Part a·Tohru HayakawaKimiya Nemoto
Jan 1, 1997·Journal of Biomedical Materials Research·T B McPhersonK Park
Jun 15, 2007·Journal of Biomedical Materials Research. Part a·Mojgan HeydariYoshihiro Ito
May 19, 2010·International Journal of Molecular Sciences·Yoshiki OshidaKoray Gençay
Jun 8, 2012·International Journal of Biomaterials·Tohru HayakawaMasao Yoshinari
Mar 27, 2012·Journal of Biomedical Materials Research. Part a·Eiji YoshidaTohru Hayakawa
Dec 9, 2003·Journal of Biomedical Materials Research. Part a·William G PittGlenn D Prestwich
Jul 15, 2005·Journal of Biomedical Materials Research. Part a·Beom Seok ParkByung-Moo Min
Apr 8, 2015·Journal of Biomedical Materials Research. Part a·Daisuke ItoYasushi Furuichi
Mar 20, 2008·Acta Biomaterialia·A BansiddhiD C Dunand

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