Chemical modification of porcine kidney aminopeptidase P indicates the involvement of two critical histidine residues

FEBS Letters
J Lim, A J Turner

Abstract

Aminopeptidase P (AP-P), purified to homogeneity from porcine kidney membranes, was completely inactivated by treatment with 0.2 mM diethylpyrocarbonate (DEP) at pH 7.0. Treatment of the modified enzyme with 20 mM hydroxylamine resulted in recovery of AP-P activity. The differential absorption of native and modified AP-P at 240 mm showed that DEP modified two histidyl residues per mol of AP-P. The substrates, bradykinin(1-5) and Gly-Pro-Hyp, and also the inhibitor, apstatin, could protect against DEP inactivation. These results suggest that histidine residues are critical for AP-P activity.

References

Mar 7, 1992·Biochimica Et Biophysica Acta·J W RyanD H Sheffy
Jun 1, 1991·European Journal of Biochemistry·H T Harbeck, R Mentlein
May 1, 1987·Analytical Biochemistry·E J HoltzmanA Yaron
Jun 1, 1987·Molecular and Cellular Biochemistry·A T OrawskiW H Simmons
Jan 1, 1974·Biochemistry·Y BursteinH Neurath
Sep 5, 1995·Biochemistry·A T Orawski, W H Simmons
Apr 1, 1995·European Journal of Biochemistry·C Vergas RomeroW Schäfer
Dec 30, 1994·Biochemical and Biophysical Research Communications·N D DenslowH P Nguyen
Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·J F BazanB W Matthews

❮ Previous
Next ❯

Citations

Mar 30, 1999·Biochimica Et Biophysica Acta·G CzirjákP Enyedi
Jan 20, 1998·Biochimica Et Biophysica Acta·D F Cunningham, B O'Connor
Jul 11, 1998·Biochimica Et Biophysica Acta·D Vines, M J Warburton

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.