Chemical, molecular, and structural characterization of alkali extractable nonstarch polysaccharides from Job's tears

Journal of Agricultural and Food Chemistry
Supaporn ApirattananusornQi Wang

Abstract

The polished Job's tears ( Coix lachryma-jobi L.) seeds, dark and white husk types, were sequentially extracted by hot water (75 degrees C) and 0.5 M NaOH solution. Nonstarch polysaccharides were not found in the water extract but were present in the alkali extract. The major components of the alkali extract from both Job's tears were protein, ash, and nonstarch polysaccharides, mainly arabinoxylans. The high arabinose to xylose ratio of 1.25 and 1.24 indicated a highly substituted structure. The average molecular weight (MW) of arabinoxylans of the dark and white husk types were 741,000 Da (Pd 1.5) and 1,449,000 Da (Pd 2.6), respectively, and their average MW reduced after treatment with protease. The alkali extractable arabinoxylans were elucidated to have a (1,4)-linked beta- d-xylan main chain highly substituted with single arabinose units. The results showed that the alpha- l-arabinofuranosyl residues (Ara f) were attached to the main chain mostly at O-3, followed by both O-2 and O-3 of xylopyranosyl residues (Xyl p).

References

Mar 30, 1992·Carbohydrate Research·R A HoffmanJ F Vliegenthart
Apr 2, 1990·Carbohydrate Research·A EbringerováM Hricovíni
Aug 1, 1973·Analytical Biochemistry·N Blumenkrantz, G Asboe-Hansen
Oct 3, 2002·Journal of Agricultural and Food Chemistry·Ching-Chuan KuoYueh-Hsiung Kuo
May 29, 2003·Journal of Agricultural and Food Chemistry·Hui-Chiu ChangWen-Chun Hung
Jun 12, 2003·Journal of Agricultural and Food Chemistry·Hsin-Yi HsuWenchang Chiang
Dec 4, 2003·Journal of Agricultural and Food Chemistry·Wen-Chun Hung, Hui-Chiu Chang
Jun 10, 2004·Biological & Pharmaceutical Bulletin·Ya-Mei YuChing-Min Tsai

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Citations

Jun 10, 2009·Carbohydrate Research·Yongzhen TaoWeilin Xu
May 5, 2009·International Journal of Biological Macromolecules·C L LinB H Chen
Dec 12, 2012·Journal of Separation Science·De-jun HuShao-ping Li
Jun 2, 2012·International Journal of Biological Macromolecules·Huihui LiuAnjun Liu
Nov 17, 2019·International Journal of Biological Macromolecules·Noura AddounGuillaume Pierre

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