Chemical Priming by Isothiocyanates Protects Against Intoxication by Products of the Mustard Oil Bomb

Frontiers in Plant Science
Elena FerberMartin J Mueller

Abstract

In Brassicaceae, tissue damage triggers the mustard oil bomb i.e., activates the degradation of glucosinolates by myrosinases leading to a rapid accumulation of isothiocyanates at the site of damage. Isothiocyanates are reactive electrophilic species (RES) known to covalently bind to thiols in proteins and glutathione, a process that is not only toxic to herbivores and microbes but can also cause cell death of healthy plant tissues. Previously, it has been shown that subtoxic isothiocyanate concentrations can induce transcriptional reprogramming in intact plant cells. Glutathione depletion by RES leading to breakdown of the redox potential has been proposed as a central and common RES signal transduction mechanism. Using transcriptome analyses, we show that after exposure of Arabidopsis seedlings (grown in liquid culture) to subtoxic concentrations of sulforaphane hundreds of genes were regulated without depletion of the cellular glutathione pool. Heat shock genes were among the most highly up-regulated genes and this response was found to be dependent on the canonical heat shock factors A1 (HSFA1). HSFA1-deficient plants were more sensitive to isothiocyanates than wild type plants. Moreover, pretreatment of Arabidopsis seedlin...Continue Reading

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Citations

Nov 14, 2021·Planta·Luciano Di FinoAna M Laxalt

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Datasets Mentioned

BETA
GSE117869
2

Methods Mentioned

BETA
BSO treatment
PCR
Chip
feature extraction
Fluorescence

Software Mentioned

package
R R Development Core
R
ImagingWin
Limma
Bioconductor
PlantGSEA

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