PMID: 6159633Aug 1, 1980Paper

Chemical probes for higher-order structure in RNA

Proceedings of the National Academy of Sciences of the United States of America
D A Peattie, W Gilbert

Abstract

Three chemical reactions can probe the secondary and tertiary interactions of RNA molecules in solution. Dimethyl sulfate monitors the N-7 of guanosines and senses tertiary interactions there, diethyl pyrocarbonate detects stacking of adenosines, and an alternate dimethyl sulfate reaction examines the N-3 of cytidines and thus probes base pairing. The reactions work between 0 degrees C and 90 degrees C and at pH 4.5--8.5 in a variety of buffers. As an example we follow the progressive denaturation of yeast tRNAPhe terminally labeled with 32P as the tertiary and secondary structures sequentially melt out. A single autoradiograph of a terminally labeled molecule locates regions of higher-order structure and identifies the bases involved.

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Citations

Jan 1, 1985·Journal of Molecular Evolution·M T MacDonell, R R Colwell
Jan 1, 1997·Journal of Biomedical Science·J.-S. ChiaP.-J. Chen
Nov 28, 2012·Journal of Biomolecular NMR·Shawn BartonMichael F Summers
May 24, 2005·Journal of Molecular Biology·Cyril GaudinDominique Fourmy
Jul 20, 2002·Journal of Molecular Biology·Alexander SerganovChantal Ehresmann
May 6, 2003·Journal of Molecular Biology·Bénédicte SohmCatherine Florentz
Jun 1, 1997·Bioorganic & Medicinal Chemistry·C S Hamann, Y M Hou
Feb 14, 2012·ACS Chemical Biology·Yogo Sakakibara, Christine S Chow
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Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D A PeattieH F Noller
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D A Peattie, W Herr
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·K KirkegaardJ C Wang
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·W Herr
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