Chemical proteomics for subcellular proteome analysis

Current Opinion in Chemical Biology
Hao ZhuItaru Hamachi

Abstract

Protein functions are tightly regulated by their subcellular localization and dynamic alteration. Chemical proteomics offers convenience and efficiency for profiling protein features in a native context. In this review, we summarize the recent progress of subcellular-compartment-focused chemical proteomics which do not rely on organelle fractionation. Organelle-specific activity-based protein profiling (ABPP) and engineered ascorbate peroxidase (APEX) have been developed for proteome analysis within organelles and even sub-organelles. In parallel, our lab designed organelle-localizable reactive molecules (ORMs) to selectively tag nuclear and mitochondrial proteins. ORMs-based proteomics is applicable to primary neurons and brain slices, as well as cultured cell lines. In addition, we invented a conditional proteomics approach to elucidate zinc homeostasis by labeling and identifying proteins localized in Zn2+-rich space of live cells.

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Citations

Apr 30, 2019·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Daniel Mauvoisin
Jun 18, 2021·Proceedings of the National Academy of Sciences of the United States of America·Yanjun LiuPeng R Chen
Feb 5, 2019·ACS Chemical Biology·Yuki NishikawaItaru Hamachi
Aug 21, 2020·Journal of the American Chemical Society·Sumin LeeChristopher J Chang

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