PMID: 882Jan 1, 1975

Chemical relaxation studies on the system liver alcohol dehydrogenase, NADH and imidazole

Acta Chemica Scandinavica. Series B: Organic Chemistry and Biochemistry
G CzerlinskiH Theorell

Abstract

Several years ago, Theorell and Czerlinski conducted experiments on the system of horse liver alcohol dehydrogenase, reduced nicotinamide adenine dinucleotide and imidazole, using the first version of the temperature jump apparatus with detection of changes in fluorescence. These early experiments were repeated with improved instrumentation and confirmed the early experiments in general terms. However, the improved detection system allowed to measure a slight concentration dependence of the relaxation time of around 3 ms. Furthermore, the chemical relaxation time was smaller than the one determined earlier (by factor 2). The data were evaluated much more rigorously than before, allowing an appropriate interpretation of the results. The observed relaxation time is largely due to rate constants in an interconversion of ternary complexes, which are faster than three (of the four) dissociation rate constants, determined previously by Theorell and McKinley-McKee.1,2 This fact contributed to earlier difficulties of finding any concentration dependence. However, the binding of imidazole to the binary enzyme-coenzyme complex can be made to couple kinetically into the interconversion rate of the two ternary complexes. The observed signa...Continue Reading

Related Concepts

Alcohol Oxidoreductases
Computers, Digital
Hydrogen-Ion Concentration
Imidazoles
Liver
Mathematics
NADH

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