Chemical Screening Using Cell-Free Xenopus Egg Extract.

Cold Spring Harbor Protocols
Matthew R Broadus, Ethan Lee

Abstract

Most drug screening methods use purified proteins, cultured cells, and/or small model organisms such as Xenopus, zebrafish, flies, or nematodes. These systems have proven successes in drug discovery, but they also have weaknesses. Although purified cellular components allow for identification of compounds with activity against specific targets, such systems lack the complex biological interactions present in cellular and organismal screens. In vivo systems overcome these weaknesses, but the lack of cellular permeability, efflux by cellular pumps, and/or toxicity can be major limitations. Xenopus laevis egg extract, a concentrated and biologically active cytosol, can potentially overcome these weaknesses. Drug interactions occur in a near-physiological milieu, thereby functioning in a "truer" endogenous manner than purified components. Also, Xenopus egg extract is a cell-free system that lacks intact plasma membranes that could restrict drug access to potential targets. Finally, Xenopus egg extract is readily manipulated at the protein level: Proteins are easily depleted or added to the system, an important feature for analyzing drug effects in disease states. Thus, Xenopus egg extract offers an attractive media for screening dr...Continue Reading

References

May 25, 1989·Nature·A W Murray, M W Kirschner
Jul 14, 1998·Molecular Cell·J WalterJ Newport
Jun 6, 2000·Journal of Biomolecular Screening·J H ZhangK R Oldenburg
Jul 6, 2004·Nature Structural & Molecular Biology·Jeffrey R PetersonMichael K Rosen
Dec 13, 2005·Methods in Enzymology·Adrian Salic, Randall W King
Feb 14, 2006·Methods in Enzymology·Andres M LebensohnMarc W Kirschner
Jun 3, 2006·Methods in Molecular Biology·Kathleen I J Shennan
Jun 3, 2006·Methods in Molecular Biology·Rene C Chan, Douglass I Forbes
Jun 3, 2006·Methods in Molecular Biology·Paula Deming, Sally Kornbluth
Jun 3, 2006·Methods in Molecular Biology·Thomas J Maresca, Rebecca Heald
Dec 3, 2008·International Journal of Cancer. Journal International Du Cancer·Igor LandaisMaureen E Hoatlin
Jan 27, 2015·Methods in Molecular Biology·Matthew R BroadusEthan Lee
Jan 18, 2017·Genesis : the Journal of Genetics and Development·Erica RaspelliVincenzo Costanzo
Jan 31, 2017·Genesis : the Journal of Genetics and Development·Jessica G BermudezMatthew C Good
Feb 14, 2018·Cold Spring Harbor Protocols·Matthew C Good, Rebecca Heald

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