Advanced solid-state NMR techniques and wet chemical analyses were applied to investigate untreated corn stover (UCS) and its residues after dilute acid prehydrolysis (DAP) and enzymatic hydrolysis (RES) to provide evidence for the limitations to the effectiveness of enzyme hydrolysis. Advanced solid-state NMR spectral-editing techniques as well as 1H-13C two-dimensional heteronuclear correlation NMR (2D HETCOR) were employed. Our results indicated that dilute acid prehydrolysis selectively removed amorphous carbohydrates, increased aromatic CH/other protonated -C═C- and enriched alkyl CH and CH2 components. Cinnamic acids were increased, and proteinaceous materials and N-containing degradation or condensation compounds were absorbed or coprecipitated in RES. 2D HETCOR experiments indicated a close association between lignin and the residual carbohydrates. Ketones/aldehydes were not detected in the DAP, in contrast to a report in which an appreciable amount of ketones/aldehydes was generated from the acid pretreatment of a purified cellulose in the literature. This suggested that acid pretreatment may modify the structure of purified cellulose more than biomass and that biomass may be a better substrate than model biopolymers a...Continue Reading
New structural information on a humic acid from two-dimensional 1H-13C correlation solid-state nuclear magnetic resonance
Nuclear magnetic resonance and diffuse-reflectance infrared Fourier transform spectroscopy of biosolids-derived biocolloidal organic matter
Elucidation of the structures of residual and dissolved pine kraft lignins using an HMQC NMR technique
Separation of aromatic-carbon 13C NMR signals from di-oxygenated alkyl bands by a chemical-shift-anisotropy filter
Ab initio molecular dynamics simulations of beta-D-glucose and beta-D-xylose degradation mechanisms in acidic aqueous solution
Absence of mobile carbohydrate domains in dry humic substances proven by NMR, and implications for organic-contaminant sorption models
Lignin depolymerization/repolymerization and its critical role for delignification of aspen wood by steam explosion
Differences between lignin in unprocessed wood, milled wood, mutant wood, and extracted lignin detected by 13C solid-state NMR
Solubilization and functionalization of sulfuric acid lignin generated during bioethanol production from woody biomass
Dissolution mechanism of crystalline cellulose in H3PO4 as assessed by high-field NMR spectroscopy and fast field cycling NMR relaxometry
Chemical structure and heterogeneity differences of two lignins from loblolly pine as investigated by advanced solid-state NMR spectroscopy
Mild acetosolv process to fractionate bamboo for the biorefinery: structural and antioxidant properties of the dissolved lignin
Differences in Cellulosic Supramolecular Structure of Compositionally Similar Rice Straw Affect Biomass Metabolism by Paddy Soil Microbiota
Solid-, solution-, and gas-state NMR monitoring of ¹³C-cellulose degradation in an anaerobic microbial ecosystem
Removal of copper and cadmium from aqueous solution using switchgrass biochar produced via hydrothermal carbonization process
Lignin removal and benzene-alcohol extraction effects on lignin measurements of the hydrothermal pretreated bamboo substrate
Controlling accumulation of fermentation inhibitors in biorefinery recycle water using microbial fuel cells.
Using RNA-Seq for gene identification, polymorphism detection and transcript profiling in two alfalfa genotypes with divergent cell wall composition in stems.
Effect of mixing on enzymatic hydrolysis of steam-pretreated spruce: a quantitative analysis of conversion and power consumption.
Cellulose digestion and metabolism induced biocatalytic transitions in anaerobic microbial ecosystems
Simultaneous and Efficient Production of Furfural and Subsequent Glucose in MTHF/H2 O Biphasic System via Parameter Regulation
Low-Temperature Synthesis of Magnetic Carbonaceous Materials Coated with Nanosilica for Rapid Adsorption of Methylene Blue
Bioinformatics in Biomedicine
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