Chemical synthesis of dual labeled proteins via differently protected alkynes enables intramolecular FRET analysis

Chemical Communications : Chem Comm
Gosuke HayashiAkimitsu Okamoto

Abstract

We report a novel method for multisite protein conjugation by setting differently silyl-protected alkynes as conjugation handles, which can remain intact through the whole synthetic procedure and provide sequential and orthogonal conjugation. This strategy enables efficient preparation of a dual dye-labeled protein and structural analysis via an intramolecular FRET mechanism.

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Citations

Apr 13, 2018·Chemical Communications : Chem Comm·Naoki KamoAkimitsu Okamoto
Aug 10, 2018·Organic & Biomolecular Chemistry·Anna KovalováMilan Vrabel
Aug 3, 2018·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Takuma SueokaAkimitsu Okamoto
Oct 3, 2017·Physical Chemistry Chemical Physics : PCCP·Jamuna K Vaishnav, Tushar Kanti Mukherjee

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