Chemical synthesis of two deoxyribododecanucleotides for the attachment of restriction termini to an artificial minigene

M S PoonianA L Nussbaum


In order to permit in vivo cloning of an artificial minigene designed to code for a modified S-peptide, the phosphodiester method for the chemical synthesis of two dodecadeoxyribonucleotides is described. Each of the latter possesses antiparallel complementarity to one of the two minigene strands and to the single-stranded EcoRI-generated end. They can thus serve as cohesive termini ("splints") for polynucleotide ligase joining.


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Related Concepts

DNA Sequence
Genes, Spliced
Genetic Code
Study, Methodological
Polynucleotide Ligases

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