Chemical tagging of chlorinated phenols for their facile detection and analysis by NMR spectroscopy

Analytical and Bioanalytical Chemistry
Carlos A Valdez, Roald N Leif

Abstract

A derivatization method that employs diethyl (bromodifluoromethyl) phosphonate (DBDFP) to efficiently tag the endocrine disruptor pentachlorophenol (PCP) and other chlorinated phenols (CPs) along with their reliable detection and analysis by NMR is presented. The method accomplishes the efficient alkylation of the hydroxyl group in CPs with the difluoromethyl (CF2H) moiety in extremely rapid fashion (5 min), at room temperature and in an environmentally benign manner. The approach proved successful in difluoromethylating a panel of 18 chlorinated phenols, yielding derivatives that displayed unique (1)H, (19)F, and (13)C NMR spectra allowing for the clear discrimination between isomerically related CPs. Due to its biphasic nature, the derivatization can be applied to both aqueous and organic mixtures where the analysis of CPs is required. Furthermore, the methodology demonstrates that PCP along with other CPs can be selectively derivatized in the presence of other various aliphatic alcohols, underscoring the superiority of the approach over other general derivatization methods that indiscriminately modify all analytes in a given sample. The present work demonstrates the first application of NMR on the qualitative analysis of the...Continue Reading

References

Aug 9, 2003·Analytical and Bioanalytical Chemistry·Martyna RompaBogdan Zygmunt
Apr 15, 2004·The Science of the Total Environment·Marianna Czaplicka
Apr 17, 2009·Environmental Science & Technology·Frances OrtonEdwin J Routledge
May 3, 2011·Environmental Science & Technology·Weiwei ZhengWeidong Qu

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