Chemical Tools and Biochemical Assays for SUMO Specific Proteases (SENPs)

ACS Chemical Biology
Yuqing JiaHuib Ovaa

Abstract

SUMOylation is a reversible and highly dynamic post-translational modification of target proteins by small ubiquitin-like modifiers (SUMO). It is orchestrated by SUMO-activating, -conjugating, and -ligating enzymes in a sequential manner and is important in regulating a myriad of predominantly nuclear processes. DeSUMOylation is achieved by SUMO-specific proteases (SENPs). Deregulation of SUMOylation and deSUMOylation results in cellular dysfunction and is linked to various diseases, including cancer. In recent years, SENPs have emerged as potential therapeutic targets. In this review, we will describe the inhibitors and activity-based probes of SENPs. Furthermore, we will summarize the biochemical assays available for evaluating the activity of SENPs to identify inhibitors.

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Citations

Feb 4, 2021·Natural Products and Bioprospecting·Jean-François GoossensChristian Bailly
May 23, 2020·Clinica Chimica Acta; International Journal of Clinical Chemistry·Yi-Zhang LiuChi Zhang
May 1, 2021·Cancers·Paulina Tokarz, Katarzyna Woźniak
Nov 26, 2020·Journal of Medicinal Chemistry·Vangelis AgouridasOleg Melnyk

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Methods Mentioned

BETA
pulldown
nuclear magnetic resonance
fluorescence resonance
FRET
Assay
Protein An assay

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