Chemical Tools for Endogenous Protein Labeling and Profiling.

Cell Chemical Biology
Kazuki ShiraiwaItaru Hamachi

Abstract

Protein analysis under biological conditions is now regarded as indispensable for understanding the structure and function of proteins, in addition to in vitro studies using purified target proteins. Because there are many molecules other than the protein-of-interest (POI) under live cell conditions, selective labeling of a POI is critical to distinguish the POI from other proteins for precise analysis. Protein labeling strategies utilizing genetically encoded tags have been used in POI modification in the complex environment of live cells. However, genetic manipulation may often induce overexpression of the POI and/or perturb the cellular context, resulting in unexpected artifacts in the protein analysis. Alternatively, recent progress in chemical biology has produced two major chemical approaches for analyzing endogenous proteins under native conditions. In this review, we summarize these techniques that utilize either protein-selective chemical labeling or proteome-directed chemical modification.

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Citations

Nov 4, 2020·Expert Opinion on Biological Therapy·Yutaka Matsuda, Brian A Mendelsohn
Jan 19, 2021·Chemical Society Reviews·Ciaran P SeathDavid W C MacMillan
Feb 6, 2021·ACS Sensors·Guoqiang Liu, James F Rusling
Feb 13, 2021·Bioscience, Biotechnology, and Biochemistry·Takeharu MinoItaru Hamachi
Apr 28, 2021·Journal of the American Chemical Society·Keita NakaneHiroyuki Nakamura
Aug 31, 2021·RSC Chemical Biology·Suprama DattaAndrew Emili
Sep 8, 2021·Chembiochem : a European Journal of Chemical Biology·Shione KamoshitaKaori Sakurai
Oct 29, 2021·Journal of the American Chemical Society·Zongyu HuangPeng R Chen
Nov 19, 2021·Chemical Communications : Chem Comm·Jordi F KeijzerBauke Albada

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