Chemical two-photon fluorescence

Analytical Chemistry
Guillermo Carrone, Roberto Etchenique

Abstract

We describe a method based on a caged fluorescent molecule that can act as a chemical two-photon probe. It is composed of an organic fluorophore and a ruthenium-bipyridine complex that acts as a photoremovable quencher. For the fluorophore to be emissive, two independent photons must act on the molecule: the first photon frees the fluorescent ligand from the Ru complex and the second photon excites the fluorescence. In this two-photon regime, the emission is not proportional to the excitation intensity but rather to its second power, as in traditional two-photon systems based on ultrashort pulsed high-power lasers. This quadratic relationship implies a much higher spatial precision on the z-axis when the probe is used in a microscopy technique. The chemical nature of the two-photon excitation mechanism allows the use of inexpensive low-power lasers.

References

Apr 6, 1990·Science·W DenkW W Webb
Oct 22, 2003·Proceedings of the National Academy of Sciences of the United States of America·Emmanuelle ChaigneauSerge Charpak
Oct 30, 2007·Nature Methods·Volodymyr NikolenkoRafael Yuste
Jun 10, 2009·Frontiers in Neural Circuits·Elodie FinoRafael Yuste
Mar 10, 2012·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Oscar FilevichRoberto Etchenique
May 16, 2013·ACS Chemical Neuroscience·Roberto ArayaRoberto Etchenique
Jan 8, 2014·Dalton Transactions : an International Journal of Inorganic Chemistry·Azadeh BahremanSylvestre Bonnet
Jul 12, 2014·The Journal of Physical Chemistry. a·Guillermo CarroneRoberto Etchenique

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Citations

Dec 26, 2015·Analytical Chemistry·Jose H HodakRoberto Etchenique
Apr 28, 2017·Chemical Communications : Chem Comm·G CarroneF Doctorovich
Feb 4, 2016·Dalton Transactions : an International Journal of Inorganic Chemistry·Alejandro CadranelJosé H Hodak
Nov 22, 2016·Dalton Transactions : an International Journal of Inorganic Chemistry·O A Lenis-RojasJ J Fernández

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