Chemical-vapor-assisted electrospray ionization for increasing analyte signals in electrospray ionization mass spectrometry

Analytical Chemistry
Zhendong Li, Liang Li

Abstract

We report a chemical-vapor-assisted electrospray ionization (ESI) technique to improve the detection sensitivity of ESI mass spectrometry (MS). This simple technique involves introducing a chemical vapor into the sheath gas around the nano-ESI spray tip or through a tubing with its outlet placed close to the spray tip. A variety of chemical vapors were tested and found to have varying degrees of effects on analyte signal intensities. The use of benzyl alcohol vapors in ESI was found to increase signal intensities of standard peptides by up to 4-fold. When this technique was combined with capillary liquid chromatography tandem MS (LC-MS/MS), the number of unique peptides identified in the acid hydrolysate of alpha casein increased by 45% and the number of peptides and proteins identified in a tryptic digest of E. coli cell lysate increased by 13% and 14%, respectively, along with an increased average match score. This technique could also increase the analyte signals for some small molecules, such as phenylephrine, by up to 3-fold. The increased analyte signals observed in the chemical-vapor-assisted ESI process is related to the enhancement of the ionization efficiency in ESI. The method can be readily implemented to an existin...Continue Reading

References

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Citations

Oct 26, 2018·Journal of the American Society for Mass Spectrometry·O Tara LiyanageElyssia S Gallagher
May 4, 2018·Analytical Chemistry·Andrea F G GarganoGovert W Somsen
Nov 18, 2020·Journal of the American Society for Mass Spectrometry·Pallab BasuriThalappil Pradeep

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