Chemically activatable viral capsid functionalized for cancer targeting

Nanomedicine
Chun-Chieh ChenR Holland Cheng

Abstract

To design a theranostic capsule using the virus-like nanoparticle of the hepatitis E virus modified to display breast cancer cell targeting functional group (LXY30). Five surface-exposed residues were mutated to cysteine to allow conjugation to maleimide-linked chemical groups via thiol-selective linkages. Engineered virus-like nanoparticles were then covalently conjugated to a breast cancer recognized ligand, LXY30 and an amine-coupled near-infrared fluorescence dye. LXY30-HEV VLP was checked for its binding and entry to a breast cancer cell line and for tumor targeting in vivo to breast cancer tissue in mice. The engineered virus-like nanoparticle not only targeted cancer cells, but also appeared immune silent to native hepatitis E virus antibodies due to epitope disruption at the antibody-binding site. These results demonstrate the production of a theranostic capsule suitable for cancer diagnostics and therapeutics based on surface modification of a highly stable virus-like nanoparticle.

References

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Citations

Sep 10, 2016·Pharmaceutical Patent Analyst·Marie Stark, R Holland Cheng
Dec 8, 2016·Nanomedicine·Jonathan Wilkinson
Apr 21, 2018·Pharmaceutical Patent Analyst·Chun C ChenR Holland Cheng
Dec 8, 2017·Scientific Reports·Marie C StarkR Holland Cheng
Nov 10, 2018·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Maria Yanqing ChenJunghae Suh
Feb 9, 2019·Scientific Reports·Eun Byul LeeSeung Kew Yoon
May 26, 2017·Biomedicines·Amelia Sadie AitkenMarie-Claude Bourgeois-Daigneault
Jan 21, 2021·Pharmaceutical Patent Analyst·Chun-Chieh ChenR Holland Cheng
Aug 15, 2019·Journal of Chemical Information and Modeling·Bo WangYinghao Wu

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Methods Mentioned

BETA
flow cytometry
fluorescence microscopy
xenografts
gel-filtration
electron microscopy
ELISA
confocal microscopy
acylation
transmission electron microscopy
xenograft

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