Chemically charging the pore constriction opens the mechanosensitive channel MscL

Biophysical Journal
K YoshimuraC Kung

Abstract

MscL is a bacterial mechanosensitive channel that protects the cell from osmotic downshock. We have previously shown that substitution of a residue that resides within the channel pore constriction, MscL's Gly-22, with all other 19 amino acids affects channel gating according to the hydrophobicity of the substitution (). Here, we first make a mild substitution, G22C, and then attach methanethiosulfonate (MTS) reagents to the cysteine under patch clamp. Binding MTS reagents that are positively charged ([2-(trimethylammonium)ethyl] methanethiosulfonate and 2-aminoethyl methanethiosulfonate) or negatively charged (sodium (2-sulfonatoethyl)methanethiosulfonate) causes MscL to gate spontaneously, even when no tension is applied. In contrast, the polar 2-hydroxyethyl methanethiosulfonate halves the threshold, and the hydrophobic methyl methanethiolsulfonate increases the threshold. These observations indicate that residue 22 is in a hydrophobic environment before gating and in a hydrophilic environment during opening to a substate, a finding consistent with our previous study. In addition, we have found that cysteine 22 is accessible to reagents from the cytoplasmic side only when the channel is opened whereas it is accessible from t...Continue Reading

References

Jan 1, 1992·Annual Review of Physiology·A S French
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B MartinacC Kung
Jan 1, 1995·Annual Review of Physiology·H Sackin
Feb 1, 1996·Neuron·H P LarssonE Y Isacoff
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·P BlountC Kung
Jan 1, 1997·Annual Review of Physiology·S I SukharevC Kung
Jan 24, 1998·The Journal of Biological Chemistry·P BlountC Kung
Sep 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·X OuC Kung
Jun 23, 1999·Structure·A F BatizaC Kung
Sep 29, 1999·The Journal of Membrane Biology·S I SukharevD R McCaslin
Jun 10, 2000·The Journal of Biological Chemistry·P C MoeP Blount

❮ Previous
Next ❯

Citations

Dec 18, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·P Koprowski, A Kubalski
Apr 7, 2005·European Biophysics Journal : EBJ·I-Jung TsaiBoris Martinac
Apr 26, 2013·Chemical Reviews·Wiktor SzymańskiBen L Feringa
Dec 31, 2011·ACS Nano·Li-Min YangPaul Blount
Jul 1, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jessica L BartlettPaul Blount
Mar 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Kenjiro YoshimuraMasahiro Sokabe
Jul 25, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jan Peter BirknerArmağan Koçer
Jul 29, 2008·The Journal of Biological Chemistry·Daniel K CrawfordRonald L Alkana
Aug 2, 2001·The Journal of General Physiology·E PerozoB Martinac
Oct 11, 2002·The EMBO Journal·Yuezhou LiPaul Blount
Mar 6, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yuezhou LiPaul Blount
Oct 12, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Li-Min Yang, Paul Blount
Apr 2, 2010·Journal of the Royal Society, Interface·Kenjiro Yoshimura, Masahiro Sokabe
Oct 18, 2008·Science·Pau Gorostiza, Ehud Y Isacoff
Feb 12, 2004·Science's STKE : Signal Transduction Knowledge Environment·Sergei Sukharev, David P Corey
Mar 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Christopher D PivettiMilton H Saier
Jun 10, 2011·PloS One·Ilja KustersArnold J M Driessen
Jul 10, 2013·Antioxidants & Redox Signaling·Boris MartinacMichael J Landsberg
Apr 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yifei KongJianpeng Ma
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ann Finney BatizaChing Kung
Apr 30, 2014·The Journal of General Physiology·Ian RoweSergei Sukharev
Aug 8, 2015·International Journal of Molecular Sciences·Ian RoweSergei Sukharev
Mar 26, 2004·Biophysical Journal·Kenjiro YoshimuraMasahiro Sokabe
Apr 2, 2003·Biophysical Journal·Giorgio ColomboAlan E Mark
Aug 4, 2015·European Biophysics Journal : EBJ·Gamma ChiMichael J Landsberg
Apr 19, 2015·Nanomedicine : Nanotechnology, Biology, and Medicine·Jesus Pacheco-TorresArmagan Kocer
Jun 6, 2006·Biophysical Journal·Grischa R MeyerBoris Martinac
Jul 21, 2001·Biophysical Journal·S SukharevH R Guy
May 23, 2002·Biophysical Journal·Iustin V Tabarean, Catherine E Morris
Nov 19, 2014·Proceedings of the National Academy of Sciences of the United States of America·Albert KonijnenbergArmağan Koçer
Apr 1, 2015·PloS One·Balasubramanian ChandramouliGiuseppe Brancato
Jan 27, 2017·Journal of the American Chemical Society·Manuel N MeloHelgi I Ingólfsson
Nov 21, 2007·Journal of Liposome Research·Armağan Koçer
May 17, 2013·Biosensors·Irene IsclaPaul Blount

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
X OuC Kung
Proceedings of the National Academy of Sciences of the United States of America
A BatizaC Kung
© 2021 Meta ULC. All rights reserved