PMID: 7021142Jan 1, 1981Paper

Chemically-induced unscheduled DNA synthesis in primary rat hepatocyte cultures: a comparison with bacterial mutagenicity using 218 compounds

Environmental Mutagenesis
G S ProbstS B Neal

Abstract

The autoradiographic identification of unscheduled DNA synthesis (UDS) in primary cultures of adult rat hepatocytes (HPC) has been proposed as a predictive test for mutagens/carcinogens. To assess the predictive value of this test, results in the hepatocyte UDS assay were compared with data for bacterial mutagenicity using a modified Ames test. Over 200 compounds representing a variety of chemical classes consisting of procarcinogens, ultimate carcinogens, and noncarcinogens were tested in each system. The accurate discrimination of many carcinogens/noncarcinogens was demonstrated by both systems. The induction of UDS in hepatocytes showed an excellent correlation with bacterial mutagenesis in response to polycyclic aromatic hydrocarbons, aromatic amines, biphenyls, nitrosamines, carbamates, azo-compounds, acridines, halogenated compounds, nitrosureas, quinolines, pyridines, purines, pyrimidines, esters and carbamates. Nitrocompounds, although active in bacteria, were poor inducers of UDS. The results support the complementary and confirmatory nature of these tests for genotoxic chemicals and indicate the usefulness of the hepatocyte UDS system as a component in a battery of short-term predictive tests for mutagens/carcinogens.

References

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Citations

Jun 1, 1987·Cell Biology and Toxicology·D A Casciano
Jun 1, 1987·Cell Biology and Toxicology·A K Thakur
Jun 1, 1987·Cell Biology and Toxicology·T R BarfknechtD J Kornbrust
Jun 1, 1987·Cell Biology and Toxicology·C A McQueen, G M Williams
Oct 1, 1984·Cell Biology and Toxicology·J A Holme, E J Søderlund
Sep 1, 1985·Archives of Environmental Contamination and Toxicology·J J Kelly, M B Maddock
May 1, 1986·Archives of Environmental Contamination and Toxicology·W R WestM L Lee
Sep 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J L Byard, K K Dougherty
Nov 1, 1986·Chemico-biological Interactions·J B Mangold, J M Lavelle
Feb 1, 1988·Mutation Research·W M Owais, A Kleinhofs
Jan 1, 1985·Pharmacology & Therapeutics·A M Jeffrey
Sep 1, 1988·Mutation Research·B Beije, L Möller
Sep 1, 1991·Mutation Research·L R Ferguson, W A Denny
Mar 1, 1993·Mutation Research·M A JacksonM D Waters
Jun 1, 1994·Mutation Research·D McGregor
Aug 1, 1992·Mutation Research·G Brambilla, A Martelli
Aug 1, 1983·Mutation Research·W G SorensonT Ong

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