Chemically modifying glass surfaces to study substratum-guided neurite outgrowth in culture

Journal of Neuroscience Methods
M MatsuzawaW Knoll

Abstract

We describe here a modification procedure for chemically fabricating neuron adhesive substrates to study the substratum-guided neurite outgrowth in culture. These substrates were fabricated by chemically attaching a synthetic peptide derived from a neurite-out-growth-promoting domain of the B2 chain of laminin. The attachment was carried out by coupling the peptide to an amine-derived glass surface using a heterobifunctional crosslinker. Hippocampal neurons were dissociated from embryonic rats and placed on the substrate at low-density in a chemically defined medium to examine the direct effect of the modified surface on their outgrowth. We observed that the neurons developed a morphology typical to that of hippocampal neurons having multiple short and single long processes within 24 h in culture. The chemical modification procedure was then combined with a UV-photo-masking technique to fabricate patterns of peptide surface on glass substrates. By culturing the hippocampal neurons on substates having alternate stripes of peptide surface and non-adhesive surface, we demonstrated substratum-controlled changes in the neuronal morphology. The modification procedure presented here can be easily achieved in the standard culture facil...Continue Reading

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Citations

Jun 6, 1998·Medical & Biological Engineering & Computing·D W BranchB C Wheeler
Feb 13, 2001·Journal of Neuroscience Methods·M SchollA Offenhäusser
Jul 9, 2002·Journal of Neuroscience Methods·Claire WyartDidier Chatenay
Apr 25, 2000·Brain Research. Developmental Brain Research·Q Y LiuW Ma
Dec 5, 1998·Brain Research. Developmental Brain Research·W MaD A Stenger
Jul 22, 1998·Progress in Neurobiology·P Heiduschka, S Thanos
Nov 10, 2001·Annual Review of Biomedical Engineering·A Folch, M Toner
May 14, 2004·Critical Reviews in Biomedical Engineering·Nianzhen LiAlbert Folch
Sep 18, 2004·Journal of Biomedical Materials Research. Part a·Nikhil O DhootMargaret A Wheatley
Mar 17, 2010·Journal of Biomedical Materials Research. Part a·Saida Khan, Golam Newaz
Jul 10, 2001·IEEE Transactions on Bio-medical Engineering·L LauerA Offenhäusser
Jun 7, 2014·Chemical Reviews·Qingjun LiuPing Wang
Mar 18, 1999·Journal of Biomechanical Engineering·B C WheelerD W Branch
Mar 25, 2006·Naunyn-Schmiedeberg's Archives of Pharmacology·Wim J E P LammersGeorg A Petroianu
Apr 11, 2000·Journal of Biomedical Materials Research·S Saneinejad, M S Shoichet
Apr 29, 1998·Journal of Biomedical Materials Research·M J IgnatiusI G Brown
Aug 27, 2008·Nanotechnology·Christelle PrinzMartin Kanje
Mar 7, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiliang LuoXinyan Tracy Cui
Feb 14, 2007·Soft Matter·Andreas OffenhäusserAngela Vogt-Eisele
Dec 13, 2006·The International Journal of Artificial Organs·K D NewmanM Griffith
Mar 20, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Waldemar HällströmMartin Kanje
Sep 21, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Girish KumarChia-Chi Ho

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