Chemiluminescence and chemotaxis assay of porcine polymorphonuclear cells. A methodological study

Zentralblatt für Veterinärmedizin. Reihe A
K Thoren-Tolling

Abstract

A Boyden-Chamber technique for leukocyte migration was adapted to a polymorphonuclear (PMN) cell concentration of 5 x 10(6) cells/ml an optimal amount of cells for the leading front technique. Casein (0.1%) is so far the most effective chemotactic substance for porcine granulocytes. A formylpeptide++, FMLP (in concentrations of 10(-2) to 10(-10) mmol/l), does not stimulate migration in porcine granulocytes when compared to random migration. For the chemiluminescence assay a concentration of 5 x 10(6) PMN cells/ml buffer is needed to obtain optimal conditions in pig granulocytes. Moreover the chemiluminescence should be performed within a standardized interval after the isolation of leukocytes since the cell activity declines rapidly after the isolation. The chemiluminescence and migration assays were standardized at 37 degrees C; the activity of the granulocytes did not show any significant variation within a temperature interval of 37-39 degrees C, whereas temperatures below 37 degrees C significantly reduce the granulocytic activity.

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Citations

May 1, 1992·Veterinary Immunology and Immunopathology·G Trowald-WighC Hård af Segerstad
Jul 1, 1996·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·A FarinelliF Indiveri
Oct 7, 2011·Centaurus; International Magazine of the History of Science and Medicine·John Henry

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