PMID: 6969212Jan 1, 1980Paper

Chemiluminescence and immune cell activation: general features of the thymocyte chemiluminescent responses to plant lectins

Immunological Communications
B K MookerjeeH Fischer

Abstract

Rat thymocytes respond to exposure to plant lectins by a burst of oxidant generation as detected by chemiluminescence (CL) (CL) in presence of luminol. All lectins tested were capable of evoking CL as long as sufficiently high concentration was used. There was no correlation between the capacity of a given lectin to evoke CL on one hand and its ability to cause mitogenic transformation or thymocyte agglutination on the other. During the first few minutes following exposure to Con A, cells in the thymocyte pool become committed to CL but once commitment is induced, removal of cell bound ligand by addition of excess methyl-alpha-D-mannoside does not significantly alter the response. Succinylated Con A is as effective as native Con A at lower concentrations but is much more effective at higher concentrations in evoking CL. Thymocyte CL can occur in absence of extracellular calcium but this calcium-independent CL is reduced by the addition of magnesium. In presence of calcium, CL response is greatly augmented and the calcium dependent response is not inhibited in presence of magnesium. The response is relatively radiosensitive. Thymocyte CL response is inhibitable by catalase and scavengers of oxidative radicals but is relatively r...Continue Reading

References

Mar 23, 1978·The New England Journal of Medicine·B M Babior
Nov 14, 1979·Biochemical and Biophysical Research Communications·E Peterhans
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·H M Korchak, G Weissmann
Jan 1, 1979·Advances in Experimental Medicine and Biology·K WrogemannH Fischer
Sep 1, 1978·Journal of Cellular Physiology·D A Hume, M Weidemann
Sep 1, 1972·Cellular Immunology·R B Whitney, R M Sutherland
Aug 1, 1970·Journal of Theoretical Biology·D E Downie
Apr 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·G R GuntherG M Edelman
Jan 1, 1973·Annual Review of Biochemistry·H Lis, N Sharon
Nov 1, 1970·Experimental Cell Research·M R Quastel, J G Kaplan

❮ Previous
Next ❯

Citations

Jun 1, 1986·Biulleten' eksperimental'noĭ biologii i meditsiny·A T SharmanovS M Kurmangaliev
Jun 1, 1995·Biological Trace Element Research·E SunJ Wang
Jan 1, 1981·Journal of Immunological Methods·E PeterhansR Wyler
Jan 1, 1982·Developmental and Comparative Immunology·B K MookerjeeI Kato

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibodies: Agglutination

Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here.