Chemiluminescence and superoxide generation by leukocytes stimulated by polyelectrolyte-opsonized bacteria. Role of histones, polyarginine, polylysine, polyhistidine, cytochalasins, and inflammatory exudates as modulators of oxygen burst

Inflammation
I GinsburgV Klimetzek

Abstract

Human blood leukocytes generate intense luminol-dependent chemiluminescence (LDCL) following stimulation by streptococci and by Gram negative rods which had been preopsonized by cationic polyelectrolytes (histone, poly L-arginine-PARG, poly L-histidine-PHSTD). Streptococci but not Gram negative rods or hyaluronic acid-rich streptococci (group C) also induced intense LDCL following opsonization with the anionic polyelectrolytes-dextran sulfate or polyanethole sulfonate (liquoid) suggesting that the outer surfaces of different bacteria bound anionic polyelectrolytes to different extents. Both normal and immune serum, synovial fluids and pooled human saliva inhibited the LDCL responses induced by streptococci preopsonized with poly cations. On the other hand, bacteria which had been first preopsonized by the various body fluids and then subjected to a second opsonization by cationic ligands ("sandwiches"), induced a very intense LDCL response in leukocytes. Streptococci which had been preopsonized by PARG, histone or by PHSTD also triggered superoxide generation by blood leukocytes, which was markedly enhanced by a series of cytochalasins. PHSTD alone induced the formation of very large amounts of superoxide. Paradoxically, the sa...Continue Reading

References

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Citations

Jan 1, 1991·Free Radical Research Communications·R KohenI Ginsburg
Nov 1, 1994·Journal of Cellular Biochemistry·S Silverton
Jun 15, 1997·Journal of Biomedical Materials Research·J K West
Jan 8, 2017·Molecular and Cellular Biochemistry·Muhammad WaqasChongsuk Ryou

Related Concepts

Polyarginine, (DL)-isomer
Serum Proteins
Cytochalasins
Histone H7
Inflammation
Leukocytes
Phosphorescent Measurements
Luminol
Macrophage
Opsonin

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