Chemiluminescence of creatinine/H2O2/Co(2+) and its application for selective creatinine detection

Biosensors & Bioelectronics
Saima HanifGuobao Xu

Abstract

Creatinine is an important biomarker in clinical diagnosis and biomonitoring programs as well as urinary metabolomic/metabonomics research. Current methods are either nonselective, time consuming or require heavy and expensive instruments. In this study, chemiluminescence of creatinine with hydrogen peroxide has been reported for the first time, and its chemiluminescence is remarkably enhanced in the presence of cobalt ions. By utilizing these phenomena, we have developed a sensitive and selective chemiluminescence method for creatinine determination by coupling with flow injection analysis. The calibration curve is linear in the range of 1×10(-7)-3×10(-5)mol/L with a limit of detection (S/N=3) of 7.2×10(-8)mol/L, which is adequate for detecting creatinine in the clinically accepted range. The relative standard deviation for seven measurements of 3×10(-5)mol/L creatinine is 1.2%. The chemiluminescence method was then utilized to detect creatinine in human urine samples after simple dilution with water. It takes less than 1min each measurement and the recoveries for spiked urine samples were 100-103%. The interference study demonstrates that some common species in urine, such as amino acids, ascorbic acid and creatine, have negl...Continue Reading

References

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Citations

Jan 13, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Suphanan SununtaNarong Praphairaksit
Oct 17, 2019·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Parveen KumarC S Pundir
Nov 20, 2019·Current Pharmaceutical Design·Yu DongTaisheng Liang
Mar 31, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Wenfeng XuPengfei Jin
Oct 16, 2016·Analytical and Bioanalytical Chemistry·Muhammad SaqibGuobao Xu
Apr 11, 2018·NPJ Digital Medicine·Jiandong WuFrancis Lin
May 13, 2021·Chemical Communications : Chem Comm·Jianyong LiChunhai Fan

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