Chemiluminescent Biosensors for Detection of Second Messenger Cyclic di-GMP

ACS Chemical Biology
Andrew B DippelMing C Hammond

Abstract

Bacteria colonize highly diverse and complex environments, from gastrointestinal tracts to soil and plant surfaces. This colonization process is controlled in part by the intracellular signal cyclic di-GMP, which regulates bacterial motility and biofilm formation. To interrogate cyclic di-GMP signaling networks, a variety of fluorescent biosensors for live cell imaging of cyclic di-GMP have been developed. However, the need for external illumination precludes the use of these tools for imaging bacteria in their natural environments, including in deep tissues of whole organisms and in samples that are highly autofluorescent or photosensitive. The need for genetic encoding also complicates the analysis of clinical isolates and environmental samples. Toward expanding the study of bacterial signaling to these systems, we have developed the first chemiluminescent biosensors for cyclic di-GMP. The biosensor design combines the complementation of split luciferase (CSL) and bioluminescence resonance energy transfer (BRET) approaches. Furthermore, we developed a lysate-based assay for biosensor activity that enabled reliable high-throughput screening of a phylogenetic library of 92 biosensor variants. The screen identified biosensors wi...Continue Reading

References

May 26, 2005·Protein Expression and Purification·F William Studier
Feb 3, 2006·Proceedings of the National Academy of Sciences of the United States of America·Thomas S ShimizuHoward C Berg
Feb 7, 2007·The Journal of Biological Chemistry·Lily I JiangPaul C Sternweis
Aug 19, 2007·Nature Medicine·Limin ZhangAlnawaz Rehemtulla
Nov 24, 2007·The EMBO Journal·Jordi BenachJohn F Hunt
Jun 24, 2008·ACS Chemical Biology·Frank FanKeith V Wood
Nov 11, 2008·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Asami KaiharaTetsushi Furukawa
Mar 17, 2009·Nature Reviews. Microbiology·Regine Hengge
Dec 8, 2009·Current Opinion in Chemical Biology·Jennifer A Prescher, Christopher H Contag
Dec 13, 2012·Nature Communications·Kenta SaitoTakeharu Nagai
Jan 8, 2013·Journal of the American Chemical Society·Chun Loong HoZhao-Xun Liang
Mar 9, 2013·Microbiology and Molecular Biology Reviews : MMBR·Ute RömlingMark Gomelsky
May 22, 2013·Proceedings of the National Academy of Sciences of the United States of America·Mitsuru HattoriTakeaki Ozawa
Sep 3, 2013·Cell·Olga Danilchanka, John J Mekalanos
May 7, 2014·Infection and Immunity·Benjamin J Koestler, Christopher M Waters
Apr 2, 2015·Proceedings of the National Academy of Sciences of the United States of America·Akira TakaiTakeharu Nagai
Jun 22, 2015·Current Opinion in Chemical Biology·Kenta Saito, Takeharu Nagai
Oct 28, 2016·Nature Communications·Jie YangCarl Hirschie Johnson
Dec 15, 2016·Nature Communications·Kazushi SuzukiTakeharu Nagai
Mar 23, 2017·Nature Chemical Biology·Petya Violinova Krasteva, Holger Sondermann
May 10, 2017·Critical Reviews in Microbiology·Elio RossiUte Römling

❮ Previous
Next ❯

Citations

Feb 16, 2020·Biophysics Reviews·Anushya PetchiappanDipankar Chatterji
Jun 16, 2021·The Journal of Experimental Medicine·Tobias Kull, Timm Schroeder
Mar 19, 2020·ACS Chemical Biology·Andrew B DippelMing C Hammond
Dec 18, 2019·ACS Sensors·Maarten MerkxMichael Jewett

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.