PMID: 16643808Apr 29, 2006Paper

Chemiluminescent detection of E. coli O157:H7 in food based on magnetic enzyme-linked immuno method

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Xiao-ying GuoZu-hong Lu

Abstract

To establish a new chemiluminescent assay with high sensitivity for detecting E. coli O157:H7 in food. The assay was established based on the alkaline phosphatase (ALP) labeled anti-E. coli O157:H7 antibody, which reacted with an excellent chemiluminescent reagent, 3(2' spiroadamantane)4 methoxy 4(3''phosphoryloxy) phenyl 1,2 dioxetane (AMPPD) buffer solution and emitted the photons. The sensitivity of chemiluminescence assay was 850, and the linear range was 1000-50,000. The intra- and interassay CV values (CVs) were respectively below 15% and 20%. The correlation coefficient was 0.9807 by comparing with the conventional counting method. The chemiluminescent immunoassay is more accurate than other methods in the detection of concentration of E. coli O157:H7 in samples.

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