PMID: 20631438Jul 16, 2010Paper

Chemiluminescent ELISA for the BTEX determination in water and soil

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
E MaioliniS Girotti

Abstract

An indirect competitive chemiluminescence enzyme-linked immunosorbent assay (CL-ELISA) for the determination of benzene, toluene, ethylbenzene, and ortho-, meta-, para-xylenes (BTEX) in soil and water was developed. The assay was optimized concerning the coating conjugate concentration, anti-BTEX antiserum dilution, incubation time effect on primary and secondary antibody incubation, and temperature effect on the competitive step and tolerance to different organic solvents. The IC(50) and lower limit of the detection (LDD(90)) values were 4.6 and 0.5 microg mL(-1), respectively. While water samples could be analyzed directly, soil has to be extracted and diluted prior to immunochemical measurements. BTEX could be recovered from spiked soil with a yield higher than 60% using 5-min ultrasonication with methanol. Finally, the assay was applied to soil and water samples collected at a contaminated site in Italy, and was compared to GC-MS.

References

Jul 19, 2003·Environmental Technology·E FrancioniM De Fátima Guadalupe Meniconi
Jun 15, 2007·Analytical Biochemistry·Harry Towbin, Otto Zingel
May 8, 2008·Clinical and Experimental Immunology·R Jafari-ShakibA Khamesipour
Jan 1, 1998·Environmental Science & Technology
Jun 22, 2013·Analytical Chemistry·N V BeloglazovaI Yu Goryacheva

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Citations

Oct 16, 2012·Analytical and Bioanalytical Chemistry·S GirottiE N Ferri

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