PMID: 7544590Jul 31, 1995Paper

Chemistry and applications of oligonucleotide analogues

Journal of Biotechnology
B S Sproat

Abstract

This review is aimed at biochemists and molecular biologists, and covers the chemistry and key features involved in the solid-phase synthesis of a variety of the better known DNA and RNA analogues by the phosphoramidite and H-phosphonate methods. A wide spectrum of biological applications such as inhibition of gene expression, translation arrest, RNA processing, affinity purification of RNA-protein complexes, in situ hybridization, and synthetic ribozymes are then discussed in some detail, enabling the molecular biologist to get an idea of what is possible using the current technology.

References

Nov 27, 1992·Science·J C HanveyK G Au
May 1, 1992·Trends in Biotechnology·S Agrawal
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·T M WoolfC G Jennings
Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·E RapaportP Zamecnik
Jul 11, 1992·Nucleic Acids Research·S M Gryaznov, R L Letsinger
Feb 25, 1991·Nucleic Acids Research·B S SproatB Beijer
Jun 1, 1991·Current Opinion in Cell Biology·A I Lamond
Feb 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·D M WilliamsF Eckstein
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·H O SmithM L Birnstiel
Feb 25, 1991·Nucleic Acids Research·J P ShawB Froehler
Apr 11, 1991·Nucleic Acids Research·R H AlulR L Letsinger
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A M IribarrenA I Lamond
Dec 25, 1990·Nucleic Acids Research·U RyderA I Lamond
Mar 1, 1990·Journal of Biochemical and Biophysical Methods·J M CampbellE Wickstrom
Jan 1, 1985·Annual Review of Biochemistry·F Eckstein
Jun 11, 1987·Nucleic Acids Research·S ShibaharaH Morisawa

❮ Previous
Next ❯

Citations

Apr 11, 2006·Organic Preparations and Procedures International·Radhakrishnan P IyerSeetharamaiyer Padmanabhan
Mar 28, 2009·International Journal of Molecular Sciences·Laura CerqueiraMaria J Vieira
Jul 5, 2005·Expert Opinion on Investigational Drugs·P J Richardson
Apr 23, 2008·Current Protocols in Nucleic Acid Chemistry·Radhakrishnan P IyerJohn E Coughlin
Aug 1, 1997·Antisense & Nucleic Acid Drug Development·T E Saison-BehmoarasC Hélène
Jun 4, 2010·Journal of the Royal Society, Interface·Adriaan M DokterIwan Holleman
Sep 24, 2004·Biochemical and Biophysical Research Communications·Jan A SikorskyJames Denvir
Aug 31, 2021·RSC Chemical Biology·Guillaume ClavéMichael Smietana

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.