PMID: 7017380Apr 13, 1981Paper

Chemistry and biology of E. coli ribosomal protein L12

Molecular and Cellular Biochemistry
N Brot, H Weissbach

Abstract

E. coli ribosomal protein L12, because of its unique features, has been studied in more detail than perhaps any of the other ribosomal proteins. Unlike the other ribosomal proteins that are generally present in stoichiometric amounts, there are four copies of L12 per ribosome, some of which are acetylated on the N-terminal serine. The acetylated species, referred to as L7, has not been shown, as yet, to possess any different biological activity than L12. A specific enzyme that acetylates L12 to form L7, using acetyl-CoA as the acetyl donor, has been purified from E. coli extracts. L12 is also unique in that it does not contain cysteine, tryptophan, histidine, or tyrosine, is very acidic (pI: 4.85) and has a high content of ordered secondary structure (approximately 50%). The protein is normally found in solution as a dimer and also forms a tight complex with ribosomal protein L10. There are three methionine residues in L12, located in the N-terminal region of the protein, one or more of which are essential for biological activity. Oxidation of the methionines to methionine sulfoxide prevents dimer formation and inactivates the protein. The four copies of L12 are located in the crest region(s) of the 50S ribosomal subunit. There...Continue Reading

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Citations

Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K ElkonN Brot
Jun 1, 1985·Archives of Biochemistry and Biophysics·D P BurmaA K Srivastava
Mar 7, 2017·Biochemistry. Biokhimii︠a︡·I V MitroshinA G Gabdulkhakov
Sep 15, 2000·Journal of Basic Microbiology·M PileckiR Szyszka
Dec 1, 1987·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·K ElkonN Brot
Nov 1, 2005·Critical Reviews in Biochemistry and Molecular Biology·Daniel N Wilson, Knud H Nierhaus
Sep 16, 2005·The Biochemical Journal·Antonella De LucaBartolo Favaloro
May 1, 2021·Frontiers in Molecular Biosciences·Laurent Aussel, Benjamin Ezraty
May 1, 1983·Archives of Biochemistry and Biophysics·N Brot, H Weissbach

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