Chemoenzymatic probes for detecting and imaging fucose-α(1-2)-galactose glycan biomarkers.

Journal of the American Chemical Society
Jean-Luc ChaubardLinda C Hsieh-Wilson

Abstract

The disaccharide motif fucose-α(1-2)-galactose (Fucα(1-2)Gal) is involved in many important physiological processes, such as learning and memory, inflammation, asthma, and tumorigenesis. However, the size and structural complexity of Fucα(1-2)Gal-containing glycans have posed a significant challenge to their detection. We report a new chemoenzymatic strategy for the rapid, sensitive detection of Fucα(1-2)Gal glycans. We demonstrate that the approach is highly selective for the Fucα(1-2)Gal motif, detects a variety of complex glycans and glycoproteins, and can be used to profile the relative abundance of the motif on live cells, discriminating malignant from normal cells. This approach represents a new potential strategy for biomarker detection and expands the technologies available for understanding the roles of this important class of carbohydrates in physiology and disease.

References

Apr 18, 1991·The New England Journal of Medicine·J S LeeW N Hittelman
Jun 12, 1998·Autoimmunity·P J Delves
Mar 26, 2003·Glycobiology·Daniel J Becker, John B Lowe
Dec 25, 2003·Journal of the American Chemical Society·Nelly KhidekelLinda C Hsieh-Wilson
Nov 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ola BlixtJames C Paulson
Dec 24, 2005·Proceedings of the National Academy of Sciences of the United States of America·Heather E MurreyLinda C Hsieh-Wilson
Apr 28, 2006·Angewandte Chemie·Joseph C ManimalaJeffrey C Gildersleeve
May 18, 2007·Journal of the American Chemical Society·Sarah R HansonChi-Huey Wong
Oct 5, 2007·Glycoconjugate Journal·Ola BlixtNahid Razi
Jan 19, 2008·Nature Chemical Biology·Jessica E RexachLinda C Hsieh-Wilson
Feb 26, 2008·Journal of the American Chemical Society·Yoshihito Tanaka, Jennifer J Kohler
Aug 8, 2008·Journal of the American Chemical Society·Peter M ClarkLinda C Hsieh-Wilson
Aug 8, 2008·Proceedings of the National Academy of Sciences of the United States of America·Wen-Wei ChangAlice L Yu
Oct 10, 2008·Journal of the American Chemical Society·Wen YiPeng George Wang
Dec 24, 2008·Proceedings of the National Academy of Sciences of the United States of America·Scott T Laughlin, Carolyn R Bertozzi
Mar 20, 2009·The New England Journal of Medicine·Fritz H SchröderUNKNOWN ERSPC Investigators
Apr 8, 2011·Angewandte Chemie·Tianqing ZhengPeng Wu

❮ Previous
Next ❯

Citations

Mar 16, 2013·Proceedings of the National Academy of Sciences of the United States of America·Nicole M OkeleyPeter D Senter
Oct 17, 2012·Glycobiology·T N C RamyaJames C Paulson
Feb 13, 2013·Molecular & Cellular Proteomics : MCP·Kevin Chandler, Radoslav Goldman
Mar 5, 2016·Cell Chemical Biology·Matthew E Griffin, Linda C Hsieh-Wilson
Apr 29, 2015·Glycoconjugate Journal·Kelly N Chuh, Matthew R Pratt
May 15, 2016·Molecular Aspects of Medicine·Larissa Krasnova, Chi-Huey Wong
Apr 14, 2016·Glycobiology·Nicole Nischan, Jennifer J Kohler
Aug 25, 2016·Journal of the American Chemical Society·Liuqing WenPeng George Wang
Dec 19, 2014·Proteomics. Clinical Applications·Radoslav Goldman, Miloslav Sanda
Jan 24, 2017·International Journal of Biological Macromolecules·Shazia TabasumKhalid Mahmood Zia
Jan 18, 2018·Chemistry : a European Journal·Xiaobao BiChuan-Fa Liu
Mar 6, 2015·Chemical Society Reviews·Li Wu, Xiaogang Qu
Oct 19, 2017·Chembiochem : a European Journal of Chemical Biology·Aime Lopez AguilarPeng Wu
Nov 19, 2014·Chembiochem : a European Journal of Chemical Biology·Sara H RouhanifardPeng Wu
Dec 24, 2014·NMR in Biomedicine·Carolyn MountfordSaadallah Ramadan
Dec 22, 2012·Chemical Society Reviews·Sara H RouhanifardPeng Wu
Apr 30, 2014·The Analyst·S DefausR Gutiérrez-Gallego
Jul 31, 2019·Glycobiology·Zhengliang L WuVassili Kalabokis
Oct 25, 2017·Beilstein Journal of Organic Chemistry·Xiao G Jia, Alexei V Demchenko
Dec 7, 2018·Journal of Carbohydrate Chemistry·Crystal L O'NeilAlexei V Demchenko
Aug 18, 2018·The Biochemical Journal·Anthony J Hayes, James Melrose
Nov 6, 2018·Chemical Science·Pierre-André GilorminiChristophe Biot
Jan 26, 2013·Chemical Society Reviews·Pavla BojarováVladimír Křen
Feb 19, 2021·The Biochemical Journal·Meg CritcherMia L Huang
Aug 10, 2020·Current Opinion in Chemical Biology·Katarzyna Radziwon, Amy M Weeks
Apr 17, 2021·Biochemical Society Transactions·Kathryn E Huxley, Lianne I Willems
May 18, 2021·IScience·Zhen LiuQiuquan Wang
Jul 28, 2021·Annual Review of Analytical Chemistry·Bo ChengXing Chen
Nov 1, 2012·Journal of the American Chemical Society·Barbara Imperiali
Oct 8, 2014·Journal of the American Chemical Society·Yuan QiaoSuzanne Walker
Jul 31, 2013·Journal of the American Chemical Society·Takahiro HayashiItaru Hamachi
Jul 17, 2018·Chemical Reviews·Liuqing WenPeng George Wang
Jun 29, 2018·Chemical Reviews·Matteo PanzaAlexei V Demchenko
Jun 18, 2014·Journal of the American Chemical Society·Arif WibowoLinda C Hsieh-Wilson

❮ Previous
Next ❯

Methods Mentioned

BETA
glycosylation
pull-down
Fluorescence
flow cytometry

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.