Chemokine expression of oral fibroblasts and epithelial cells in response to artificial saliva

Clinical Oral Investigations
Heinz-Dieter MüllerReinhard Gruber

Abstract

Artificial saliva is widely used to overcome reduced natural salivary flow. Natural saliva provokes the expression of chemokines in oral fibroblasts in vitro. However, if artificial saliva changes the expression of chemokines remains unknown. Here, we investigated the ability of Saliva Orthana®, Aldiamed®, Glandosane®, and Saliva Natura® to change the expression of chemokines in human oral fibroblasts and the human oral epithelial cell line HSC-2 by means of reverse transcription polymerase chain reaction and immunoassays. Mucins isolated from bovine submaxillary glands and recombinant human mucin 1 were included in the bioassay. Formazan formation and LIVE/DEAD® staining determined the impact of artificial saliva on cell viability. The involvement of signaling pathways was determined by pharmacologic inhibitors and Western blotting. In gingival fibroblasts, Saliva Orthana®-containing mucins provoked a significantly increased expression of CXC ligand 8 (CXCL8, or interleukin 8), CXCL1, and CXCL2. Immunoassays for CXCL8 and CXCL1 confirmed the translation at the protein level. The respective dilution of artificial saliva had no impact on formazan formation and LIVE/DEAD® staining. Mucins isolated from bovine submaxillary glands ...Continue Reading

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Citations

Jan 20, 2017·Clinical Oral Investigations·Heinz-Dieter MüllerReinhard Gruber
Jun 16, 2021·Cell·Drake Winslow WilliamsNiki Maria Moutsopoulos
Dec 2, 2021·Journal of Biomedical Materials Research. Part a·Yosuke AsamiYasutomo Yajima

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