Chemokine-triggered microtubule polymerization promotes neutrophil chemotaxis and invasion but not transendothelial migration

Journal of Leukocyte Biology
Sandeep Kumar YadavRonen Alon

Abstract

Microtubules (MTs) are critically involved in the transport of material within cells, but their roles in chemotactic leukocyte motility and effector functions are still obscure. Resting neutrophils contain few MTs assembled in an MT organizing center (MTOC) behind their multilobular nuclei. Using a probe of real-time tubulin polymerization, SiR-tubulin, we found that neutrophils elongated their MTs within minutes in response to signals from the two prototypic chemotactic peptides, CXCL1 and fMLP. Taxol, a beta-tubulin binding and MT stabilizing drug, was found to abolish this CXCL1- and fMLP-stimulated MT polymerization. Nevertheless, taxol treatment as well as disruption of existing and de novo generated MTs did not impair neutrophil protrusion and squeezing through IL-1β-stimulated endothelial monolayers mediated by endothelial deposited CXCL1 and neutrophil CXCR2. Notably, CXCL1-dependent neutrophil TEM was not associated with neutrophil MT polymerization. Chemokinetic neutrophil motility on immobilized CXCL1 was also not associated with MT polymerization, and taxol treatment did not interfere with this motility. Nevertheless, and consistent with its ability to suppress MT polymerization induced by soluble CXCL1 and fMLP, ta...Continue Reading

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Citations

Jul 21, 2020·ELife·Cristina Peligero-CruzJakub Abramson
Aug 20, 2019·Essays in Biochemistry·Melina Michael, Sonja Vermeren
Feb 27, 2021·Frontiers in Cell and Developmental Biology·Aglaja Kopf, Eva Kiermaier
Oct 20, 2020·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Xinyuan ZhangKeyue Shen
Sep 9, 2021·Journal of Leukocyte Biology·Wei LiuZhichao Fan

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