Chemokines and integrins independently tune actin flow and substrate friction during intranodal migration of T cells

Nature Immunology
Miroslav HonsMichael Sixt

Abstract

Although much is known about the physiological framework of T cell motility, and numerous rate-limiting molecules have been identified through loss-of-function approaches, an integrated functional concept of T cell motility is lacking. Here, we used in vivo precision morphometry together with analysis of cytoskeletal dynamics in vitro to deconstruct the basic mechanisms of T cell migration within lymphatic organs. We show that the contributions of the integrin LFA-1 and the chemokine receptor CCR7 are complementary rather than positioned in a linear pathway, as they are during leukocyte extravasation from the blood vasculature. Our data demonstrate that CCR7 controls cortical actin flows, whereas integrins mediate substrate friction that is sufficient to drive locomotion in the absence of considerable surface adhesions and plasma membrane flux.

References

Oct 1, 1990·The Journal of Cell Biology·D F KucikM P Sheetz
Feb 19, 1988·Science·D Bray, J G White
Feb 9, 1996·Cell·T J Mitchison, L P Cramer
Feb 7, 1998·The Journal of Experimental Medicine·R A WarnockU H von Andrian
Jun 29, 2002·Cellular Immunology·B C SchaeferR M Kedl
Aug 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Joachim C U LehmannAlf Hamann
Feb 28, 2007·The Journal of Experimental Medicine·Tim WorbsReinhold Förster
Feb 28, 2007·The Journal of Experimental Medicine·César Nombela-ArrietaJens V Stein
Apr 28, 2007·Trends in Immunology·Tim Worbs, Reinhold Förster
Aug 25, 2007·Nature Reviews. Immunology·Klaus LeySussan Nourshargh
May 3, 2008·Nature·Tim LämmermannMichael Sixt
May 24, 2008·Nature·Kinneret KerenJulie A Theriot
Jun 10, 2008·Nature Methods·Julia RiedlRoland Wedlich-Soldner
Feb 10, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jordan JacobelliMatthew F Krummel
Feb 26, 2009·Genes & Development·Kyle R LegateReinhard Fässler
Nov 17, 2009·Nature Cell Biology·Jörg RenkawitzMichael Sixt
Mar 3, 2010·Nature Methods·Julia RiedlRoland Wedlich-Söldner
Sep 28, 2010·Blood·Mustapha FaroudiVictor L J Tybulewicz
May 12, 2011·PLoS Biology·Erin L BarnhartJulie A Theriot
May 21, 2011·Nature Reviews. Immunology·Nancy HoggFrances Willenbrock
Dec 14, 2011·Proceedings of the National Academy of Sciences of the United States of America·Noa OferKinneret Keren
Mar 23, 2012·PLoS Computational Biology·Meghan K DriscollWolfgang Losert
Jul 3, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tomoya KatakaiTatsuo Kinashi
Feb 11, 2015·The Journal of Cell Biology·William A ComrieJanis K Burkhardt
Feb 14, 2015·Cell·Verena RuprechtCarl-Philipp Heisenberg
Mar 17, 2015·Nature Cell Biology·Martin BergertEwa K Paluch
Jun 18, 2015·The New England Journal of Medicine·Kerry DobbsLuigi D Notarangelo
Nov 19, 2015·Frontiers in Immunology·Jens V Stein
Jan 30, 2016·Physical Review Letters·A C Callan-JonesR Voituriez
Feb 9, 2016·Nature Reviews. Immunology·Matthew F KrummelAudrey Gérard
Oct 11, 2016·Nature Communications·Pontus NordenfeltTimothy A Springer
Sep 5, 2017·Cell·Jan MuellerMichael Sixt
Dec 13, 2017·Nature Communications·Pontus NordenfeltTimothy A Springer

❮ Previous
Next ❯

Citations

May 20, 2018·Nature Immunology·Patrick W Oakes, Deborah J Fowell
Apr 13, 2019·Immunological Reviews·Guilhem R ThierryMarc Bajenoff
Apr 13, 2019·Immunological Reviews·Tim Lämmermann, Wolfgang Kastenmüller
Apr 13, 2019·Immunological Reviews·Nadine EckertReinhold Förster
Apr 13, 2019·Immunological Reviews·Jens V Stein, Nora Ruef
Oct 5, 2019·Nature Reviews. Molecular Cell Biology·Kenneth M Yamada, Michael Sixt
Jan 3, 2020·The EMBO Journal·Sudha KumariDarrell J Irvine
Mar 24, 2020·PLoS Biology·Candelas Álvarez-SalameroMaría N Navarro
Nov 5, 2019·Frontiers in Immunology·Silvia DusiBarbara Rossi
Jun 26, 2020·Nature·Anne ReversatMichael Sixt
Nov 9, 2018·Frontiers in Immunology·Mario NovkovicBurkhard Ludewig
Dec 29, 2020·Frontiers in Immunology·Karl-Gösta Sundqvist
Aug 21, 2020·Molecular Aspects of Medicine·Juho VuononvirtaThanushiyan Poobalasingam
Feb 26, 2021·Nature Reviews. Immunology·Deborah J Fowell, Minsoo Kim
Mar 30, 2021·Frontiers in Immunology·Michael J Shannon, Emily M Mace
Mar 4, 2020·Journal of Cell Science·Hélène D MoreauPaolo Pierobon
Sep 1, 2019·Journal of Cell Science·Michael J ShannonDylan M Owen
Jul 23, 2020·Journal of Cell Science·Xuan LuoOlivier Theodoly
Apr 21, 2021·Nature Materials·Kolade AdebowaleOvijit Chaudhuri
Jun 22, 2021·Frontiers in Immunology·Andrea M AmitranoMinsoo Kim
May 23, 2021·Biophysical Journal·Inge M N WortelJohannes Textor
Jun 17, 2021·American Journal of Physiology. Cell Physiology·Hao SunZhichao Fan
Sep 4, 2020·Biophysical Journal·Laurene AounOlivier Theodoly
Sep 28, 2021·Frontiers in Immunology·Flavian ThelenJens V Stein
Nov 18, 2021·Immunological Reviews·Dominik Schienstock, Scott N Mueller
Dec 3, 2020·Biophysical Journal·Zeinab SadjadiHeiko Rieger
Nov 20, 2021·The FEBS Journal·Veneta Gerganova, Sophie G Martin

❮ Previous
Next ❯

Methods Mentioned

BETA
GTPases
confocal microscopy
fluorescence recovery after photobleaching
FCS
atomic
AFM

Software Mentioned

XlStat
Volocity
Live Acquisition
JPK
GraphPad
Prism
Matlab

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.