Chemolabile cellular microarrays for screening small molecules and peptides

Molecular Diversity
Antje HoffRoland Brock

Abstract

Microarrays that mediate the uptake of small molecules into living cells are described. Tissue culture cells were seeded onto glass substrates functionalized locally with fluorescently labelled test substances. In order to enable a localized transfer of substances after contact of cells with the substrate, substances were immobilized on the surface either by non-covalent interactions or chemolabile linker groups. These chemolabile linker groups were incorporated into covalently immobilized compounds. Different ester linkages were evaluated as chemolabile linker groups. As model compounds, esters of the carboxy group of a cysteine with the hydroxy groups of carboxyfluorescein-labelled serine amide and tyrosine amide residues or the thiol group of another fluorescein-labelled cysteine amide were generated. Covalent immobilization occurred on maleimide-functionalized glass cover slips. The surface functionalization and release kinetics were assessed by confocal laser scanning microscopy. The fastest release was obtained for the phenolic tyrosine ester. Alternatively, fluorescently labelled peptides were immobilized by non-covalent interactions on glass and on a hydrogel matrix. In order to increase the efficiency of cellular uptak...Continue Reading

Citations

Jun 1, 2010·Cancer Immunology, Immunotherapy : CII·Antje HoffRoland Brock
Aug 15, 2012·Biotechnology Journal·Drew M TitmarshJustin J Cooper-White
Jun 24, 2006·Drug Discovery Today·David CastelXavier Gidrol
Sep 10, 2005·Expert Review of Molecular Diagnostics·Brigitte Angres
Jun 12, 2013·Chemical Communications : Chem Comm·Kenji UsuiHisakazu Mihara

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.