Chemometric and Transcriptomic Profiling, Microtubule Disruption and Cell Death Induction by Secalonic Acid in Tumor Cells.

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Nadire ÖzenverThomas Efferth

Abstract

Nature is an indispensable source of new drugs, providing unique bioactive lead structures for drug discovery. In the present study, secalonic acid F (SAF), a naturally occurring ergochrome pigment, was studied for its cytotoxicity against various leukemia and multiple myeloma cells by the resazurin assay. SAF exhibited cytotoxic activity on both leukemia and multiple myeloma cells. Generally, multiple myeloma cells were more sensitive to SAF than leukemia cells. NCI-H929 cells were the most affected cells among the tested panel of multiple myeloma cell lines and were taken for further studies to assess the mode of action of SAF on those cells. Cell cycle analysis revealed that SAF induced S and G2/M arrest in NCI-H929 cells. SAF-associated apoptosis and necrosis resulted in cytotoxicity. SAF further inclined the disassembly of the tubulin network, which may also account for its cytotoxicity. COMPARE and hierarchical cluster analyses of transcriptome-wide expression profiles of the NCI tumor cell line panel identified genes involved in numerous cellular processes (e.g., cell differentiation, cell migration, and other numerous signaling pathways) notably correlated with log10IC50 values for secalonic acid. In conclusion, the pre...Continue Reading

References

Nov 1, 1979·Journal of Toxicology and Environmental Health·C S ReddyA Ciegler
Jan 21, 1977·The Journal of Organic Chemistry·R AndersenA L Demain
Jul 1, 1974·Chemical & Pharmaceutical Bulletin·M HaradaK Miyaki
Jan 1, 1971·Chemical & Pharmaceutical Bulletin·M YamazakiK Miyaki
Jan 1, 1983·Cancer Chemotherapy and Pharmacology·M ShimizuT Iwaguchi
Dec 5, 1998·Science·J N Weinstein
Mar 4, 2000·Nature Genetics·U ScherfJ N Weinstein
Apr 2, 2004·Nature Reviews. Cancer·Mary Ann Jordan, Leslie Wilson
Feb 24, 2006·Archives of Pharmacal Research·Hong RenWeiming Zhu
Oct 5, 2010·Nature Reviews. Drug Discovery·Charles Dumontet, Mary Ann Jordan
Dec 15, 2010·Pharmacogenetics and Genomics·Connie OshiroRuss Altman
Mar 18, 2011·The New England Journal of Medicine·Antonio Palumbo, Kenneth Anderson
Oct 18, 2011·Seminars in Cell & Developmental Biology·Irina Kaverina, Anne Straube
Dec 3, 2014·The Lancet Oncology·S Vincent RajkumarJesus F San Miguel
Apr 10, 2015·Marine Drugs·Lilia CherigoSergio Martinez-Luis
Feb 9, 2016·Journal of Natural Products·David J Newman, Gordon M Cragg
Jun 11, 2016·International Journal of Hematology·Shinsuke Iida
Jun 12, 2016·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Victor KueteThomas Efferth
Jun 14, 2016·American Journal of Hematology·S Vincent Rajkumar
Sep 7, 2016·Progress in Tumor Research·Gunnar Juliusson, Rachael Hough
Oct 1, 2016·Circulation Research·Jun-Ichi AbeEdward T H Yeh
Jul 1, 2017·Future Cardiology·Robert J Henning, Raymond D Harbison
Jul 1, 2017·Blood Cancer Journal·T Terwilliger, M Abdul-Hay
Mar 15, 2018·Frontiers in Pharmacology·Mohamed E M SaeedHeba Abdel-Aziz
Nov 25, 2018·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Devesh TewariPawan Kumar Singh
Jan 27, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lulu XieMingdong Zhao

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Citations

Nov 4, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Aveen N AdhamThomas Efferth

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Methods Mentioned

BETA
Assay
flow cytometry
Fluorescence Microscopy

Software Mentioned

GraphPad Prism
bioinformatic COMPARE
COMPARE
GraphPad
Excel

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