Chemonucleolysis with human stromelysin-1

Spine
H HaroK Shinomiya

Abstract

Immunohistologic analysis was performed on surgically removed samples of herniated nucleus pulposus to examine the expression of stromelysin-1. We performed in vitro and in vivo experiments to determine whether recombinant human (rh) stromelysin-1 is capable of degrading nucleus pulposus. To analyze the production of stromelysin-1 in various types of herniated nucleus pulposus, and to examine the effects of this recombinant protein on nucleus pulposus tissues. The authors previously demonstrated a progressive decrease in herniated nucleus pulposus size in some of the transligamentous and sequestration types of herniated nucleus pulposus using magnetic resonance imaging. An increased production of stromelysin-1, a cartilage proteoglycan degrading enzyme, in herniated nucleus pulposus was reported recently. The authors speculated that if stromelysin-1 is involved in the degradation of herniated nucleus pulposus, stromelysin-1 itself may be used as a chemonucleolytic agent. Immunohistologic analysis using streptoavidin-biotin method was performed on 20 herniated nucleus pulposus samples to investigate the expression of stromelysin-1. Five herniated nucleus pulposus samples were incubated in a tissue culture medium in the presence ...Continue Reading

References

Apr 1, 1992·Spine·W J Gogan, R D Fraser
Jul 1, 1991·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·J LiuJ S Mort
Sep 24, 1990·Biochimica Et Biophysica Acta·S MasureG Opdenakker
Aug 1, 1989·The Journal of Clinical Investigation·D D DeanJ F Woessner
Feb 4, 1988·The New England Journal of Medicine·J W Frymoyer
Mar 1, 1986·Spine·M D Brown, J S Tompkins
Mar 1, 1987·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E Vila-PorcileC Tougard
Oct 1, 1984·Journal of Neurosurgery·J ArtigasH M Mayer
Feb 1, 1995·Arthritis and Rheumatism·L J BonassarA J Grodzinsky
Jun 29, 1963·Nature·L SMITHR B JENNINGS

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Citations

Jun 1, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Hirotaka HaroKenichi Shinomiya
Jan 26, 2002·Joint, Bone, Spine : Revue Du Rhumatisme·L GrangR Juvin
May 25, 2002·Joint, Bone, Spine : Revue Du Rhumatisme·Michel Benoist
Dec 28, 2010·Journal of Neurosurgery. Spine·Andreas ZigourisAthanasios P Kyritsis
Mar 4, 2008·Journal of Neurosurgery. Spine·Hirotaka HaroYoshiki Hamada
Jan 8, 1998·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·H HaroK Shinomiya
Mar 1, 2005·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Hirotaka HaroDan M Spengler
Jun 15, 2007·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Masanori WakoYoshiki Hamada
Sep 20, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Koji FujitaHirotaka Haro
Jun 21, 2005·Biomaterials·Sadahiro IwabuchiHirotaka Haro
Dec 4, 2013·The Spine Journal : Official Journal of the North American Spine Society·Hirotaka HaroHiromichi Komori

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